Effect Of Mesenchymal Stem Cells On Liver Injury Protection And SIRT1 Gene Expression In Newly Diagnosed Type 1 Diabetic Mice

Type 1 diabetes mellitus(T1DM)is an autoimmune disease which is regulated by T cells.Stem cell therapy is a new promising method for T1 DM treatment.Objective: To observe the effect of treatment of diabetic liver injury in T1 DM mice and explore its possible mechanism.Methods: After diagnosing T1 DM,the experimental mice were randomly divided into the following groups.(1)Diabetic group: no any interventions after the onset of the disease.(2)Glargine group: treated with Glargine;(3)h UCMSCs group: treated with h UCMSCs.Body weight and the blood glucose was measured.HE staining was used to observe the liver lesions.ELISA was used to detect the expression of AGEs.Real-time PCR was used to detect m RNA expression of RAGE? P65?IL-6 and TNF? in liver tissue.Western blot was used to detect the expression of SIRT1 protein in liver tissues.Results:(1)After the treatment,the blood glucose level in the glargine group and the h UCMSCs treatment group was significantly lower than that in the diabetic group.(2)HE staining revealed that the liver tissue organization of the diabetic group,hepatic lobule was not clear,hepatocytes were ill-defined and swell,arrangement of hepatic cords was disordered,obvious inflammatory cell infiltration was observed.Compared with the T1 DM group,the mesenchymal stem cells group andglargine groupwere significantly improved.(3)Compared with diabetic group,expression of AGEs in the mesenchymal stem cells group andglargine groupwere significantly lower(P<0.05).(4)Compared with diabetic group,RAGE,P65,IL-6 and TNF? expressions in the mesenchymal stem cell group andglargine groupwere significantly lower(P<0.05).(5)Compared with diabetic group,SIRT1 in h UCMSCs treated group andglargine group had up-regulated expression(P<0.05).Conclusions:(1)Mesenchymal stem cells reduced the diabetic liver damage.(2)The protective effects of mesenchymal stem cells in diabetic liver mainly depended on reducing inflammatory factors and increasing the expression of SIRT1.