Study On The Relationship Between Serum Thyroid Hormone Levels And VEGF Expression In Villi Tissue

Background: In recent years,the incidence of embryo termination is increasing year by year,the etiology is complex,and the pathogenesis is not clear.More and more studies have shown that vascular epithelial growth factor(cardiovascular,VEGF)plays an important role in the maintenance of early pregnancy.It has been found that thyroid hormone(TH)can regulate the synthesis and secretion of important proteins,enzymes and lipids in brain tissue,such as myelin basic protein,myelin associated protein,protein lipid protein,calcium binding protein,luminosity phosphate receptor,neurological / RC3 and so on.It plays an important role in the development and maturation of brain tissue.Thyroid hormone deficiency can cause clinical symptoms such as mental retardation,motor disorders and paralysis.Especially in the early pregnancy,the development of fetal brain tissue is very sensitive to the level of thyroid hormone.Thyroid hormone deficiency is related to a variety of adverse pregnancy outcomes,such as embryo termination,spontaneous abortion,placental abrupt,stillbirth,fetal growth restriction,preterm delivery and so on.However,there are few reports on whether there is a correlation between the abnormal expression of VEGF and TH and embryo termination.Objective: To investigate the correlation between serum thyroid hormone level and VEGF expression in chorea villi in patients with embryo termination,so as to provide a theoretical basis for the mechanism of embryo termination.Methods: From November 2018 to July 2019,67 patients with embryo arrest and normal pregnancy treated in French District Maternal and Child Health Hospital of Dining City,Shannon Province were divided into embryo termination group and normal pregnancy group(Normal early pregnancy requires termination of pregnancy).All patients were selected into the group in strict accordance with the inclusion criteria and exclusion criteria,and all patients were terminated by artificial abortion.Serum trinitrotoluene(trinitrotoluene T3)and radiotelephone(T4)levels were measured by luminescence method in all patients at 7-8 weeks and 9-10 weeks of gestation.The serum levels of trinitrotoluene(trinitrotoluene T3)and radiotelephone(T4)were measured by luminescence method.The chorea villi were taken from 10 min after induced abortion and washed with normal saline to remove blood.After paraffin embedding and tissue sections,the expression level of VEGF in chorea villi of each group was detected by photochemical method.All the operations were approved by the Medical Ethics Committee of French District Maternal and Child Health Hospital in Dining City,Shannon Province,and the statistical software SPSS20.0 was used to analyze the dataobtained in the experiment.The levels of serum T3 and T4 and the expression of VEGF in chorea villi were compared by independent sample t-test.The correlation between the levels of serum T3 and T4 and the expression of VEGF in chorea villi was analyzed by Pearson correlation analysis.Results:(1)The levels of serum T3,T4 and VEGF in the embryo termination group were significantly lower than those in the normal pregnancy group(P < 0.05);(2)There was a positive correlation between the serum levels of T3 and T4 and the expression of VEGF in the chorea villi(P < 0.05);(3)There was no correlation between the levels of serum T3 and T4 and the expression of VEGF in the chorea villi in the normal pregnancy group.Conclusion: There is a correlation between the level of serum thyroid hormone and the expression of VEGF in chorea villi in patients with embryo termination.The decrease of thyroid hormone level may affect the expression of VEGF in chorea villi,which is one of the possible causes of embryo termination.