Study On The Relationship Between The Changes Of Plasma Cytokine Profile And The Rapid Progress And Long-term Non Progress Of HIV Infection

Objective: The disease progression of human immunodeficiency virus((HIV))infection is different,and cytokines play a key role in the disease progression of HIV virus infection.HIV virus infection will induce the increase of cytokine secretion and promote immune activation,forming a "cytokine storm".Recent studies have shown that "cytokine storm" is closely related to the progression of HIV infection.At present,most studies are mainly focused on the cytokines of patients with rapid progression of HIV infection,and there are also reports on cytokines of patients with long-term non-progression of HIV infection.It has not been reported whether the bond cytokines can not only affect the rapid progression of the disease,but also be related to long-term non-progress.The purpose of this study is to find the key cytokines that play a role in the rapid progression and long-term non-progression of HIV-infected diseases,to find clinical markers of important value,and to provide new clues for disease intervention.Methods: In this study,the plasma samples of 79 patients with HIV infection in the Red Ribbon Clinic of the first affiliated Hospital of China Medical University were selected,including 22 fast progressives,19 long-term non-progressives and 38 typical progressives.At the same time,20 healthy adults were recruited as the control group.The changes of 27 kinds of cytokines were detected by Bio-Plex Cytokine kit.At the same time,to study the key cytokines that play a role in the rapid progression and long-term non-progression of HIV-infected diseases,to clarify the relationship between them and the progression of HIV-infected diseases,and to explore the relationship between cytokine networks of different types of disease progression.Results:1.Changes of cytokine spectrum between HIV infected persons and normal persons: to study the differences of plasma cytokine profiles between HIV infected persons(rapid progressives,typical progressives)and normal subjects,it was found that 9 kinds of cytokines changed significantly after infection,including IL-1ra(p < 0.001),IL-9(p=0.003),IL-13(p= 0.028)and so on.Compared with normal subjects,14 kinds of cytokines in long-term non-progressives and typical progressives changed significantlyafter infection,including IL-1 ?(p < 0.01),IL-4(p < 0.017),IL-5(p < 0.001),IL-6(p <0.010)and so on.2.The difference of cytokine spectrum in patients with different disease progression of HIV infection: it was found that there were 11 kinds of cytokines changed between the rapid progressive group and the typical progressive group,including IL-7,IL-8,IL-9,IL-13,Eotaxin,IP-10,MCP1,MIP1 a,MIP1 ?,PDGF-bb,GM-CSF.Compared with the typical progression,there were 15 kinds of cytokines that changed in the long-term non-progressives,including IL-1 ?,IL-4IL-5,IL-6,IL-7,IL-8,IL-9,IL-10,IL-12,IL-17,IP-10,G-CSF,IFN-?,TNF-?.Among them,IP-10 increased in those who made rapid progress and decreased in those who did not progress for a long time.3.The relationship between IP-10 and the progression of HIV infection: to further explore the correlation between plasma IP-10 level and the number of CD4+T cells and viral load in patients with HIV infection.It was found that the level of plasma IP-10 in patients with rapid progression of the disease was negatively correlated with the number of CD4+T cells in patients with HIV infection(r=-0.529,p=0.001).The level of plasma IP-10 in patients with long-term non-progression of the disease was also negatively correlated with the number of CD4+T cells in patients with HIV infection(r=-0.450,p=0.002).The plasma IP-10 level of patients with rapid progression of disease was positively correlated with the viral load of HIV infection(r=0.377,p=0.023),while the plasma IP-10 level of patients without long-term disease progression was positively correlated with the viral load of HIV infection(r=0.300,p=0.051).4.There were differences in the correlation between plasma cytokines in patients with HIV infection with different disease progression: the number of associations between single cytokines in patients with rapid progression was significantly lower than that in patients with typical progression(300).There was a significant difference in the correlation between the two groups of infected persons(p=0.035).The number of associations of single cytokines in long-term non-progressives was 199,which was significantly higher than that of typical progressives(154).There was a significant difference in the correlation between the two groups of infected cytokines(p=0.028),and the cytokine network of long-term non-progressives was closer.Conclusion: There were significant differences in cytokine profiles among HIV infectedpatients with different types of disease progression.Among the changed cytokines,IP-10 was the only cytokine that increased in the rapid progression of the disease and decreased in those who did not progress for a long time,and the increase of IP-10 level was related to the decrease of CD4+T cells and the increase of viral load in infected patients.It is suggested that IP-10 is an important index to predict the rapid progression of the disease,and may provide a new target for delaying the disease progression of HIV-infected people.