Study On The Etiology Of Patients With Diabetes Complicated By Pulmonary Infection

Objective:(1)Retrospective investigation of the distribution characteristics of pathogenic bacteria in lung infections in patients with diabetes mellitus(DM),providing reference basis for early empirical treatment of the disease.(2)Prospectively collected sputum samples from patients with DM and pulmonary infection for multi-pathogen and multi-fluid nucleic acid detection.To evaluate the clinical application and value of multi-pathogen multi-flux nucleic acid detection method for the diagnosis of bacterial pathogens in patients with DM and pulmonary infection.Methods:(1)Select 843 clinical data of DM patients with community-acquired pneumonia treated in multiple hospitals in Shenyang from January 2018 to December2019,collect relevant etiology data,and analyze the pathogenic bacteria composition.(2)A total of 104 sputum specimens from patients with DM and pulmonary infection in our hospital from January 2018 to June 2019 were collected,and multi-pathogen and multi-fluid nucleic acid tests were performed.The test results were analyzed and compared with common bacterial cultures to evaluate their clinical application.Result:(1)In the 843 cases,92 cases of sputum bacteria were cultured with pathogenic bacteria and 137 strains were cultured,among which gram-negative bacteria were the most common,accounting for 47.5,and the detection rate of A.baumannii was increased.Gram-positive bacterial infections accounted for 25.5.Except for Streptococcus pneumoniae,Grape bacteria accounted for a relatively high proportion of about 8.4,and positive results of fungal culture accounted for 27.(2)The positive rate of multi-pathogen multi-fluid nucleic acid detection was 71.2(74/104),and the positive rate of bacterial culture was 32.7(34/104),the difference was statistically significant(P <0.05).Multi-pathogen multi-flux nucleic acid detection improves the detection rate of difficult-to-cultivate bacteria.Conclusion:(1)When lung infection occurs in patients with DM,it is recommended thatantibiotics combined with anti-infective treatments that are effective against gram-negative bacilli should be selected,and pathogenic specimens should be actively taken early to select sensitive antibiotics for targeted treatment based on drug sensitivity tests.(2)Multi-pathogen multi-fluid nucleic acid detection technology can quickly diagnose bacterial pathogens,which can help clinicians quickly identify the types of bacterial pathogens with DM combined with lung infections,reduce the use of unnecessary antibacterial drugs,and reduce the generation of drug-resistant strains.