The Effect Of MiR-193a-3p On Cell Proliferation And Apoptosis In H9C2 Rat Cardiomyocytes

Objective: Embryonic heart development and occurrence of congenital heart disease are regulated by a variety of different genetic factors,among which T-box factor 1(TBX1)and transforming growth factor-?(TGF-?)signaling pathway have already been proved to be indispensable.Our previous studies have found that down-regulated expression of TBX1 gene may regulates the expression of TGF-?2 through inhibiting the expression of miR-193a-3p.However,how this pathway performs on cellular level and its effect on cell function remains unclear.Since studies have shown that plenty of miRNAs regulate related biological activities and participate in pathogenesis of diseases through affecting cell proliferation and apoptosis,this paper presents research on the effect of miR-193a-3p on proliferation and apoptosis in rat cardiomyocytes H9C2,so as to explore the possible mechanism of this pathway involved in heart development and congenital heart disease.Method:(1)MiR-193a-3p mimics and miR-193a-3p inhibitor was transfect separately into H9C2 cells,after which transfection efficiency was detect by qRT-PCR.(2)After miR-193a-3p over-expression and inhibition,proliferation of cardiomyocytes were evaluated by CCK-8.(3)Apoptosis rate of H9C2 cells was measured by flow cytometry 48 hours post-transfection,using Annexin V-FITC / PI double-staining.(4)Experimental data were analyzed by SPSS19.0 software,and P<0.05 was considered with statistical significance.Result:(1)Compared with negative control,the proliferation of rat H9C2 cells was significantly inhibited after miR-193a-3p over-expression(P<0.01).While after miR-193a-3p inhibition,on the contrary,cell proliferation was promoted(P<0.05).(2)Compared with negative control,48 hours after miR-193a-3p mimics transfection,the apoptosis rate of H9C2 cells showed an increase(P<0.05).When transfected with inhibitor,in the opposite way,apoptosis rate reduced(P<0.05).And during these two processes,effects on early apoptosis were more significant than those of advanced stages.Conclusion: MiR-193a-3p downregulates the proliferation of rat cardiomyocytes H9C2 while upregulates their apoptosis,and the regulation of apoptosis might happen in its early stage.This could be the possible way of miR-193a-3p involved in heart development,and altered miR-193a-3p level may contribute to congenital heart disease through inhibiting cell proliferation and promoting cell apoptosis.