Study On The Quality Of Cistanche Herba,Qianliguang Gao And Meconopsis Horridula

With the continuous improvement of people's recognition and our countrythe's attention,ethnomedicine has a good development prospects.Because of the variety of Chinese herbal medicines,along with their complex composition and the uneven quality of the same medicinal materials in the market,the development of ethnomedicine is seriously hindered.The study on the chemical composition and quality standardization of ethnomedicine is helpful to clarify the material basis of effective substances and provide reference for the comprehensive evaluation of the quality.This dissertation consists of three chapters,which was about the quality research of three ethnomedicines.The first chapter reported the determination of phenylethanoid glycosides in Cistanche herba by HPLC.In the second chapter,the quality standard of Qianliguang Gao was established,including TLC identification,the content determination of moisture,ash and principal component.The last chapter reported the content determination method of flavonoids in Meconopsis horridula.The first chapter reported the content determination of echinacoside,cistanoside A,verbascoside and 2-acetylacteoside in Cistanche tubulose and Cistanche deserticola by HPLC.This chapter also reported the content change of four phenylethanoid glycosides in Cistanche deserticola from different regions and different growth periods.The content of echinacoside and verbascoside in Cistanche tubulose was higher than that in Cistanche deserticola.The content of these four phenylethanolic glycosides in Cistanche tubulose from Inner Mongolia was higher than that from Xinjiang.For the content of these four phenylethanoid glycosides in different growth periods of Cistanche deserticola from Inner Mongolia,echinacoside content prior to anthesis was more than haustorium and more than florescence.Cistanoside A content of haustorium was more than prior to anthesis and more than post anthesis.Verbascoside content prior to anthesis was more than post anthesis and more than the haustorium.2-acetylacteoside content post anthesis was more than prior to anthesis and more than the haustorium.The second chapter was to study the quality of Tibetan medicine Qianliguang Gao and establish the quality standard.The first was to identify chlorogenic acid by TLC.The results of TLC showed that 10 batches Qianliguang Gao were rich in chlorogenic acid.Secondly,moisture content was determined by Karl Fischer titration method and drying method.According to the general rule 2302 ash determination method of Chinese Pharmacopoeia?2015 edition?,total ash and acid insoluble ash content were determined.Finally,chlorogenic acid in the sample solution was enriched using SPE and the content was determined by HPLC.The results showed that the average content of water,total ash and acid insoluble ash was 3.04%,23.18%and 3.16%,respectively.The average content of chlorogenic acid was 24.9727 mg?g^-1.These methods were quick,convenient and reliable,which could be used for quality control of Qianliguang Gao.In the third chapter,the extraction method,content determination method,and classified quantitative analysis of flavonoids and their glycosides in a Tibetan herb-Meconopsis horridula were established by HPLC.Firstly,free flavonoid aglycones in four batches of Meconopsis horridula were determined by HPLC.The content of luteolin and apigenin was 0.0947 mg?g^-1 and 0.1597 mg?g^-1,respectively.Then,the quantitative methods of flavonoids after acid hydrolyzing were established.The content of luteolin,kaempferol and apigenin handled by acid hydrolysis method was 0.1507 mg?g^-1,0.1526 mg?g^-1 and 0.1238 mg?g^-1,respectively.Comparing the content change of flavonoid aglycones in two methods,the content of aglycones for relevant glycosides for luteolin and kaempferol was0.0560 mg?g^-1 and 0.1526 mg?g^-1,respectively.Through the comparison of flavonoid content before and after hydrolysis,the luteolin,kaempferol,apigenin and their corresponding flavonoid glycosides could be quantified effectively.The interference of flavonoids with similar structure and the difficulty to obtain trace components were avoided,and the content profile of flavonoids in this herb could be grasped conveniently.