Preparation Of Strontium Doped Sol-gel Bioactive Glass And Porous Bone Prosthesis

Bioactive glass has good biocompatibility and osteoinductivity,can activate the expression of bone-related genes,and has a good bone regeneration and repair function.In recent years,it has received great attention from the biomedical materials research field.Due to their homogeneous chemical composition,special micro nano structure and high specific surface area,the sol gel bioactive glass shows better bioactivity and tissue repair properties than the fused bioactive glass,and has good application prospects.In order to obtain a better bone repair effect,this paper adds strontium?Sr?,an important trace element in the human body,on the basis of Ca O-P₂O₅-Si O₂ three-component sol-gel bioactive glass,in order to improve the material in the tissue Immune control function during repair.At the same time,according to the principle of bionic structure,the porous bone prosthesis?scaffold?was prepared.Studies have shown that this kind of bone restoration with multi-level pore structure is conducive to the regeneration and repair of bone defects.This three-dimensional porous bone repair scaffold can provide an ideal three-dimensional space for the proliferation and directional differentiation of osteoblasts,and has a very good role in promoting the formation of new bone.In this paper,strontium-doped Sr O-Ca O-P₂O₅-Si O₂ quaternary system sol-gel bioactive glasses with good biological activity were prepared,and by designing different strontium-doped control groups,their in vitro mineralization ability,as well ability to repair bone and promote angiogenesis was systematically evaluated.An organic foam template method was used to prepare a bioactive glass porous bone prosthesis with interconnected porous structure,and its physical and chemical properties,in vitro apatite forming ability,in vitro degradation and cell adhesion properties were investigated.The main research work and conclusions are as follows:?1?Hydrochloric acid is used as a catalyst to successfully prepare sol-gel bioactive glass powder,which is micron-sized particles and has a high specific surface area and a large mesopore structure.At the same time,it has good biological activity and can be quickly formed by contact with simulated body fluids abundant apatite deposits.?2?Based on the preparation method of sol-gel bioactive glass,Sr O-Ca O-P₂O₅-Si O₂quaternary system strontium doping sol-gel bioactive glass powder with different strontium content is prepared,a certain amount of strontium content substituted for part of calcium will not change its particle size,phase composition and chemical structure;It still has good hydroxyapatite formation ability and ion dissolution.With the increase of strontium content,the release rate of Si and Ca ions is accelerated,the degradation rate of bioactive glass is increased,and the formation ability of apatite mineralization on the surface of the material is reduced;Cytological experiments showed that it had good biocompatibility,and could significantly promote the proliferation of mouse bone marrow mesenchymal stem cells?m BMSCs?and human umbilical vein endothelial cells?HUVECs?,and enhance the expression of osteoblast markers?ALP?and angiogenic markers?CD31?of HUVECs.The bioactive glass doped with an appropriate amount of Sr?3 mol%,3Sr-SBG group?can better promote cell survival,osteogenic differentiation and vascular differentiation.?3?The organic foam template method was used to prepare a bioactive glass porous bone restoration with a connected porous structure.The prepared bone restorations all have connected multi-level pores,Multi-stage porous materials with micro-meso and macro pores have moderate strength and porosity up to 82%,It has good hydroxyapatite formation ability and strong anti-collapsing ability.Cell experiments verified that it can promote the rapid adhesion of m BMSCs.