Studies On Iridoids And Their Bioactivities From Valeriana Jatamansi Jones

Three chapters make up this thesis.The first chapter was a review about the research progress on both the chemical constituents and their pharmacological activities of Valeriana jatamansi Jones;the iridoids from the root and rhizome of V.jatamansi and their bioactivities were discussed in the second chapter;conclusion of this dissertation was made in the third chapter.Valeriana jatamansi Jones,belonging to the Family Valerianaceae,is an annual herb distributed widely in the southwest of China.This plant contains a variety of constituents,such as iridoids,sesquiterpenes,volatile oils,lignans and flavones.V.jatamansi is a traditional Chinese medicine used for the treatment of various diseases,including nervous disorders,epilepsy,insanity,skin diseases,and so on.As the main constituents of V.jatamansi,the iridoids possessed sedative,antianxiety effects,along with anti-virus,anti-tumor,anti-inflammatory,antibacterial activities,and so on.Therefore,we discussed the isolation and structural elucidation of the isolates.In addition,the anti-influenza,anti-inflammatory and anti-glioma stem cell activities were also evaluated.Systematical studies on the chemical components and biological activities of the roots and rhizomes of V.jatamansi were carried out.By the means of all kinds of chromatographic methods,such as silica gel,Sephadex LH-20,MCI,MPLC and HPLC,along with various spectroscopic techniques,including MS and NMR,46 iridoids were isolated from the title plant.Among them,seven iridoids were new ones,which were identified as jatadoid C?1?,patriscadoid??2?,patriscadoid??3?,jatadoid D?4?,chlorovaltrate X?5?,chlorovaltrate Y?6?and chlorovaltrate Z?7?.The known iridoids were identified as valeriandoid C?8?,jatairidoid C?9?,jatamanin U?10?,jatamanin O?11?,jatamanin S?12?,?1S,3R,5R,7S,8R,9S?-1,5-dihydroxy-3,8-epoxy-valechlorine?13?,jatamanin?14?,?1S,3R,5S,7S,8S,9S?-1-methoxy-7-hy droxy-8-methyl-3,8-epoxy-?^4,11-dihyron-petane?15?,?3S,4S,5S,7S,8S,9S?-3,8-ethoxy-7-dihydr-oxy-4,8-dimethlperhydrocyclopenta[c]pyran?16?,4-acetoxy-3-hydroxy-8-isovaleroxy-l0-methy len-2,9-dioxatricyclo?4.3.l.03,7?decan?17?,jatamanvaltrate B?18?,valeriotriates B?19?,chlorovaltrate H?20?,jatamanvaltrate E?21?,valeriotetrate C?22?,jatamanvaltrates G?23?,jatamanvaltrates H?24?,IVHD-valtrate?25?,jatamanvaltrate L?26?,jatamandoid A?27?,baldrinal?28?,11-methoxyviburtinal?29?,deacylbaldrinal?30?,isovaltrate?31?,deacetylisovaltrate?DI??32?,10-isovaleroxy-valtrathydrin?33?,jatamanvaltrate Q?34?,valeriandoid F?35?,isovaltrate acetoxyhydrin?36?,jatamanvaltrate K?37?,rupesin B?38?,patriscabrin C?39?,patriscadoid II?40?,jatamanvaltrate W?41?,patriscadoid I?42?,valeriandoid D?43?,valjatrate E?44?,valjatrate G?45?and 8,9-didehydro-7-hydroxy-dolichodial?46??Anti-influenza virus,anti-inflammatory and antitumor activities in vitro of the isolated iridoids were also evaluated.As a result,compound 36 showed obvious anti-influenza virus activity,with IC₅₀ value of 19.26?M,while compounds 31 and 42 exhibited moderate anti-influenza virus activities.Compounds 33-35,37,41 and 45 significantly inhibited the production of NO in RAW 264.7 induced by LPS,with IC₅₀ values of 5.04,5.57,0.88,0.62,4.07and 9.75?M,respectively.Moreover,compound 35 could inhibit the growth of human glioma stem cells GSC-3#and GSC-18#,with IC₅₀ values of 3.25 and 2.61?M.At the same time,it could also hinhibit the growth of human glioma cells U251 and U87,with IC₅₀ values of 15.49and 18.41?M.