| Purpose Cancer as the second leading cause of death in human beings,among which the incidence of digestive tract tumors in the first in each system,Colorectal cancer(CRC)as one of the most common tumors in the digestive tract,The incidence of CRC is increasing day by day..The prognosis of gastrointestinal tumors depends on the stage of the disease.White light endoscopy(WLE)examination has the possibility of missed diagnosis and misdiagnosis,and biopsy can lead to bleeding,causing submucosal fibrosis and adhesion,affecting the subsequent endoscopic treatment.Image enhanced endoscopy(IEE)technology only emphasized to observe from the perspective of morphology and diagnosis of gastrointestinal disease,the lack of the tumor target recognition.Molecular imaging(MI)is a new field that is revolutionizing cancer diagnosis and treatment.As a combination of basic science and clinical endoscopy,molecular imaging is emerging as a new direction of medical and scientific research.Near-infrared fluorescence(NIRF)imaging is a branch of Optical Molecular Imaging,which has the advantages of high spatial and temporal resolution,no radiation,portability and economy.NIRF imaging has been applied to cancer detection,imaging of sentinel lymph nodes and intraoperative navigation of tumor surgery.Combined with targeted fluorescent probes to the characteristics of specific molecular targets to achieve high specificity tag,build suitable for digestive tract tumor detection the wide field of near-infrared fluorescence molecular endoscopy is of great significance in the diagnosis of digestive tract tumors.The purpose of this study is to find an safe,portable,economical,real-time and efficient method to achieve in a wide field near-infrared fluorescence molecular endoscopy imaging of gastrointestinal tumors.Methods 1.Using high resolution fiber bund le,2000 lx/high sensitivity MKC F16-700hd camera,LED light source,the 24-inch screen,commercial optical accessories,building near infrared fluorescent molecular endoscopic imaging equipment,and test the imaging equipment,the system resolution,sensitivity for fluorescence probe.Detection of near-infrared fluorescence molecular endoscopic imaging device in a clinical environment.(1)Construction of mouse model of subcutaneous colon cancer tumor transplantation and detection of near-infrared fluorescence molecular imaging effect:Mouse model of subcutaneous tumor transplantation for colon cancer: tumor cells of MC38 colon were injected into the forelimb of nude mice and the tumor formation was observed.(2)Detection of imaging effects of rodent colon cancer model by near-infrared fluorescence molecular endoscopy.Using the tail vein injection of Bevacizumab-IRDye800 CW for optical imaging,observe the device for wide-field near-infrared molecular imaging of colon cancer tumor-burdened rat and mouse model of orthotopic transplantation tumor effect.2.The application and research of near-infrared fluorescent molecular endoscopic imaging equipment:(1)Construction of a mouse model of colon cancer subcutaneous transplantation tumor: MC38 colon tumor cells were inoculated under the forelimb of the mouse and the results of tumor formation was observed.(2)Detection of the effect of near-infrared fluorescent molecular endoscopic imaging equipment on mouse colon cancer model.The ICG-Cetuximab probe was injected into the tail vein for optical imaging to observe the wide-field dual-channel near-infrared fluorescence molecular imaging effect of the device in the colon cancer subcutaneous transplantation t umor and orthotopic transplantation mouse models,and was combined with micro PET / CT comparison.Results 1.Successfully developed a set of wide-field dual channel near-infrared fluorescent molecular endoscopy imaging equipment.The optical design and parameter components are discussed in detail.Ikegami's s COMS camera was used in the near-infrared fluorescent molecular imaging device,with 2.2m pixel 1/3 inch F16 2000lux sensitivity scanning the CMOS sensor line by line,achieving a signal to noise ratio of 56 d B and a high resolution of 1000 TVL.AVI video and JPEG images of 1092 x 1080 pixels are available.A standard resolution target was used to test the resolution of the near-infrared fluorescent molecular endoscopic imaging device.The dual-channel imaging fiber has a resolution of 6.25 microns after detection,and a principle prototype is constructed.2.The device's specifications and imaging effects can meet the ergonomic needs of the current clinical environment of digestive endoscope centers and are compatible with digestive endoscopes.3.Using MC38 to successfully construct a nude mouse subcutaneous xenograft model.A model of colon cancer subcutaneous transplantation tumor was injected with 200 u L ICG-Cetuximab probe in the tail vein of nude mice,and a significant fluorescent signal could be seen after subcutaneous transplantation.Observe the dual-channel wide-field imaging system based on the fluorescence imaging effect of optical fiber.MC38 was used to successfully construct a mouse orthotopic colon cancer xenograft model.The ICG-Cetuximab probe was injected into the tail vein to observe the white light and real-time near-infrared fluorescent molecular imaging of in situ tumors,and there was inflammatory interference in small animal PET / CT.Conclusion 1.Successfully built a near-infrared fluorescent molecular endoscopy with a diameter of 3mm,including a light source,light guide-imaging fiber and probe,camera and information processor,and video surveillance system.Combined with existing digestive endoscopy.2.Animal in vivo imaging studies show that the endoscopic imaging system can clearly image colon cancer subcutaneous and orthotopic tumors,and achieve wide field imaging of colon cancer lesions.3.The equipment has the advantages of no radiation,reduced detection cost,and small equipment f ootprint.Wide-field endoscopic detection of gastrointestinal tumors and precancerous lesions provides a new imaging method. |
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