Mechanism Study About The Role Of CCL23-CCR1 Axis In The Progression Of Epithelial Ovarian Cancer

Background and aims: This study was designed to investigate the effect of CCL23-CCR1 chemokine axis in the progression of human epithelial ovarian cancer diseases,and do some relevant preparatory exploration about the role of CCL23-CCR1 in epithelial ovarian cancer chemotherapy resistance.Methods: The levels of 441 cytokines in serum of 6 patients with epithelial ovarian cancer and 4 normal adult females were detected using Rio Biotech antibody array.56 differentially expressed proteins were screened and one of the differentially expressed chemokine CCL23 was selected as our research object.Serum levels of CCL23 in 22 normal adult females and 36 epithelial ovarian cancer patients were detected by ELISA.q RT-PCR was used to detect the expression of CCL23 m RNA in 6 normal adult ovarian tissues and 6 epithelial ovarian cancer tissues.Western blot was used to detect the expression of CCL23 protein;Immunohistochemistry was used to detect the expression of CCR1 in situ lesions of 20 cases of Stage ?,20 ?,40 ? and 20 Stage ? epithelial ovarian cancers.Result: The levels of 441 cytokines in serum of 6 patients with epithelial ovarian cancer and 4 normal adult females were detected using antibody array and CCL23 concentration in normal control group was(2192.42±264.78)pg/ml;CCL23 concentration in patients with epithelial ovarian cancer was(4490.20±206.40)pg/ml,t=-2.17,P=0.03,the difference was statistically significant.Serum levels of CCL23 in 22 normal adult women were(1157.80±457.40)pg/ml;serum levels of CCL23 in 36 cases of epithelial ovarian cancer patients were(1654.90±849.00)pg/ml,t=-2.443,P=0.0188,the difference was statistically significant.The expression of CCL23 m RNA in epithelial ovarian cancer tissue was significantly higher than that in normal ovarian tissue,t=-12.95,P<0.001.The protein expression of CCL23 in epithelial ovarian cancer group was significantly higher than that in normal control group.The expression of CCR1 in situ lesions of epithelial ovarian cancer was detected using immunohistochemistry,demonstrating that ovarian surface epithelial(OSE)presented negative expression of CCR1.In contrast,epithelial ovarian cancer group presented positive or strong positive expression.Average optical density of CCR1 in 60 cases of stage ?/?(1.77±0.46)was higher than 40 cases of stage ?/?(0.85±0.35),the difference was statistically significant(t=-4.403,P=0.001).Conclusions: 1.Serum concentration of CCL23 in epithelial ovarian cancer group was higher than that of the normal control group.3.CCR1,the CCL23`s cell membrane receptor,showed high expression in ovarian epithelial ovarian cancer group.Moreover,CCR1 exhibited higher expression in phase III/IV ovarian cancer than phase I/II,also strongly suggesting that CCL23 may be involved in the progression of epithelial ovarian cancer.