Influenza Virus Activates TLR7/NF-?B Signaling Pathway To Regulate Airway Inflammatory Response In Patient With AECOPD

Objective: To explore the molecular mechanism of influenza A virus activating TLR7/NF-kappa B signaling pathway in regulating acute exacerbation of airway inflammation in COPD,and to provide a new target and theoretical basis for clinical treatment of IAV infection induced AECOPD.Methods: Isolation,purification,identification,and culture of human bronchial epithelial cells after obtaining normal and COPD patients' bronchial tissue from surgical specimens.The experiment was divided into NHBE group,NHBE + IAV group,DHBE group and DHBE + IAV group.After 24 hours of co-culture with IAV,they were co-cultured with TLR7 si RNA.The expressions of TLR7 and NF-?B in epithelial cells were detected by Western blot.The contents of interleukin-6 and tumor necrosis factor-? were detected by enzyme-linked immunosorbent assay.Results: Compared with group A,TLR7 protein expression in group B,group C and group D was 0.350±0.075 in group A,0.950±0.075 in group B,0.780±0.056 in group C,1.280±0.031 in group D,NF-?B protein expression in group A(0.470±0.034 in group A,1.090±0.078 in group B,0.910±0.045 in group C,1.540±0.051 in group D),and IL-6 level in group A(53.000±6.532 in group A,185.000±7.874 in group B.TNF? level in group A was significantly increased(17.000±1.625 in group A,8.000±0.526 in group B,29.000±1.323 in group C,52.000±3.453 in group D)(all P values were <0.01);TLR7,NF-?B protein expression and IL-6,TNF? were significantly increased in group D compared with group C(P<0.01).Compared with group A,TLR7(0.530±0.023 in group A,0.350±0.047 in group E),NF-B(0.800±0.046 in group A,0.510±0.067 in group E),il-6(51.000±0.327 in group A,26.000±1.081 in group E),TNF leukin(14.000±0.314 in group A,8.000±0.526 in group E),TNF leukin(P<0.05)were significantly lower in group E than in group A.Compared with group C,TLR7(group C 1.080±0.078,group F 0.880±0.056),NF-B(group C 1.280±0.034,group F 1.040±0.029),il-6(group C 125.000±2.249,group F 83.000±1.125),TNF PCR(group C 28.000±1.010,group F 21.000±0.429)decreased(P<0.05).Conclusions: Influenza viruses activate TLR7/NF-kappa B signaling pathway to regulate airway inflammation storms in patients with acute exacerbation of COPD.The results provide a theoretical basis for clinical search for new therapeutic targets of AECOPD induced by influenza viruses.