The Effect Of MYEOV On Proliferation And Migration Of Human Pancreatic Cancer Cells And Its Mechanism

Objective: In this study,the expression of MYEOV and its association with survival and prognosis were analyzed by the bioinformatics methods in pancreatic cancer data sets,to investigate the effect of MYEOV knockdown on the proliferation and migration of human pancreatic carcinoma cell line SW1990 in vitro,and to further explore its possible mechanism,with a view to discovering a potential molecular target for the diagnosis,prognosis assessment and treatment of pancreatic cancer.Methods: The expression level and clinical value of MYEOV were analyzed in TCGA,GTEx and CCLE data,and tested in Oncomine database.And then the expression of MYEOV m RNA and protein in pancreatic cells was detected by Real-time PCR and Western Blot.Further two lentivirus-mediated sh RNAs were constructed which targeting MYEOV and infected SW1990 cells to obtain SW1990-sh1 and SW1990-sh2 experimental groups,while the blank plasmid was transfected as a negative control group and the untreated cells as a blank control group.The proliferation of cells was determined by CCK-8 assay;the cell migration ability was analyzed by scratch test;The m RNA expression of SMADs in the TGF-? signaling pathway was detected by Real-time PCR.Results: 1.Bioinformatics analysis showed that MYEOV overexpressed in pancreatic cancer in TCGA and CCLE databases,and its high expression was related to poor clinical prognosis in pancreatic cancer patients,while it was low expressed in normal pancreatic tissues in GTEx database.2.In vitro cell experiments showed that MYEOV overexpressed in 6 pancreatic cancer cells,compared with the pancreatic ductal epithelium cell line HPDE6(P<0.01).However,low level expression of MYEOV protein was detected only in PANC-1 and SW1990 cells.Constructed stable knockdown MYEOV SW1990 cells successfully.Compared with the control groups,the expression of MYEOV m RNA decreased in experimental groups(P<0.001).The proliferation rate and migration of SW1990 with MYEOV knockdown were lower than the control groups(P<0.001).Compared with the control groups,the m RNA expression of SMAD1,SMAD4,SMAD5 and SMAD9 decreased in the experimental groups as a result of down-regulation of MYEOV,while the expression of SMAD2,SMAD3 and SMAD7 m RNA was only down-regulated in the SW1990-sh2 group(P<0.001).Conclusion: MYEOV overexpresses in pancreatic cancer,compared with the adjacent and normal pancreatic tissues,and high expression of MYEOV is associated with poor clinical prognosis in pancreatic cancer patients.MYEOV m RNA is overexpressed in pancreatic cancer cells.MYEOV could promote the proliferation and migration of pancreatic cancer cells via TGF-?/SMAD pathway in vitro.Therefore,MYEOV is expected to be a potential molecular target for the diagnosis,prognosis assessment and treatment of pancreatic cancer.