The Application Of New Magnetic Nanoparticles In The Separation Of Killer T Cells

The application of magnetic nanotechnology has promoted the development of various fields,especially in biomedical research and clinical fields.As a new cancer treatment method,immune cell therapy has attracted more and more attention in recent years due to its good therapeutic effect.In the research of immune cell therapy,the separation of immune cells is particularly important,and the sorting technology can influence the quality of the required immune cells.Magnetic nanoparticles have many advantages such as strong magnetic response,good biocompatibility,and large specific surface area.Scientists h ave found that magnetic nanoparticles have important potential application value in cell separation.In order to better study the magnetic nanoparticles which can be used in the separation of tumor killing T-lymphocytes,this project uses the new magnetic nanoplatform built in the laboratory,and selects two kinds of magnetic nanoparticles,carboxy magnetic beads and amino magnetic beads,for CD8 specific antibody coupling experiment.The coupling effect of this two kinds of magnetic nanoparticles on antibody is obtained by BCA method.It is found that the coupling effect of amino magnetic beads is better than that of carboxy magnetic beads under the condition of the same antibody dosage.Further,through the gradient experiment of antibody dosage,the coupling amount of the antibody on the surface of the amino magnetic bead is studied,the result shows that when the ratio of antibody to the magnetic bead is 50 μg: 3 mg,the maximum coupling rate of the amino magnetic beads is 96.65%.The experiment also uses Nicomp380/ZLS to analyze the particle size before and after the coupling reaction between the magnetic beads and the antibody.The results show that the average particle size and polydispersity of the immune magnetic beads have increased to varying degrees,indicating that the coupling of antibody can influence the dispersion and average particle size of immune beads,and it is found that the dispersion of the amino immune beads is better than that of the carboxyl immune beads under the same conditions,which further proves that amino magnetic beads are more suitable for the research of this project than carboxy magnetic beads.The purpose of this project is to study the magnetic nanoparticles that can be used to separate killer T cells,Through the study o f the selection of magnetic nanoparticles,the amount of antibody coupling and the properties of immune magnetic beads,we can draw the conclusion that the magnetic nanoparticles have reference value in the study of CD8+T lymphocytes separation,and provides a theoretical basis for the subsequent study of cell separation with magnetic nanoparticles and CD8+T lymphocyte immunotherapy.