Effects Of 40Hz Light On Various Types Of Hippocampal Cells In Stroke Mice And Its Neuroprottective Mechanism

Stroke is the number one health killer in China and the number one cause of life loss in China.When a blood clot blocks or blocks an artery to the brain,the blood vessels lose oxygen and nutrients to the brain tissue behind them,and an ischemic stroke occurs.Strokes eventually involve the death and dysfunction of brain cells,as well as neurological defects that reflect the location and size of damaged brain areas.In the past decade,significant advances have been made in the treatment and prevention of stroke.Many studies have emphasized the basic mechanism of irreversible damage to brain cells after a stroke.Research has focused on what predisposes neurons to death and what strategies allow them to resist ischemic damage.It has been found that drive 40 Hz gamma-wave oscillations may induce an overall neuroprotective response.Two-vessel occlusion model was used in this paper,immunohistochemistry was used to compare the differences of hippocampal microglia,astrocytes,neurons,interneurone and newborn neurons in the brains of mice exposed to 40 Hz light after stroke and to investigate the neuroprotective effect of 40 Hz light on the brain after ischemic stroke.In combination with in vitro culture of neurons,to explore the inhibitory apoptosis proteins expression,to find a new way of neuronal protection and explore a new scheme of stroke treatment.Study shows that 40 Hz light can relieve the inflammation of microglia and astrocytes of brain in mice after stroke effectively,increase the number of newborn neurons in the brain,repair the damaged nervous system and can also promote the antiapoptotic proteins prothymosin ?,inhibit further apoptosis and further damage of the nervous system caused by cell necrosis after tissue ischemia.In vivo,prothymosin ? will spread to surrounding cells,which can resist the damage caused by ischemic stroke to some extent.Unlike traditional surgical treatments,light treatment can also alleviate damage,promote brain regeneration and protect brain tissue function.Stimulating the expression of apoptotic inhibitor to inhibit the brain tissue necrosis is expected to be a new method for the treatment of nerve injury.