| Objective: To explore the relationship between the expression levels of CDK7,SRp20,SF3B3 and clinicopathological characteristics in ovarian cancer(especially serous carcinoma).To investigate the pathogenesis and progression mechanism of ovarian serous carcinoma(OSC)and to identify new therapeutic targets in case to provide novel choice for the treatment of ovarian cancer.Method: One hundred and ten cases of OSC,26 cases of serous borderline tumor(SBT),28 cases of serous cystadenoma(SC)and 30 cases of fallopian tube were selected.Among the 110 OSCs,81 were high-grade serous carcinoma(HGSC)and 29 were lowgrade serous carcinoma(LGSC).The expression levels of CDK7,SRp20 and SF3B3 in these cases were detected by immunohistochemistry.Analyzing the differences in the expression of CDK7,SRp20 and SF3B3 in different groups,and exploring the association between the expression levels of CDK7 and SRp20,SF3B3 in OSC.Searching for the relationship between the expression levels of CDK7,SRp20,SF3B3 and the clinicopathological characteristics of OSC.Survival analysis was performed in OSC patients.In addition,CDK7,SRp20 and SF3B3 were also detected in 27 cases of ovarian clear cell carcinoma(OCCC)and 23 cases of ovarian endometrioid carcinoma(OEC)at the same time.The expression results were compared with those of OSC,so as to investigate the expression difference of the 3 indicators in different types of ovarian carcinoma.Results: 1.The expression of CDK7 in normal fallopian tube tissues and serous tumors: The high expression rates of CDK7 in normal fallopian tube tissues,SC,SBT,LGSC,HGSC were 0.0%(0/30),0.0%(0/28),3.9%(1/26),10.3%(3/29),72.8%(59/81).The expression differences of CDK7 in the groups of normal fallopian tube tissues / SC and SBT,LGSC,HGSC,and the groups of SBT / LGSC and HGSC were statistically significant(all P < 0.05),while in normal fallopian tube tissues and SC,SBT and LGSC were not statistically significant(both P > 0.05).2.The expression of SRp20 in normal fallopian tube tissues and serous tumors: The high expression rates in normal fallopian tube tissues,SC,SBT,LGSC,HGSC were 0.0%(0/30),0.0%(0/28),3.8 %(1/26),10.3%(3/29),71.6%(58/81).The expression differences of SRp20 in the groups of normal fallopian tube tissues / SC and SBT,LGSC,HGSC,and the groups of SBT / LGSC and HGSC were statistically significant(all P < 0.05),while in the fallopian tube tissues and SC,SBT and LGSC were not statistically significant(both P > 0.05).3.The expression of SF3B3 in normal fallopian tube tissue and serous tumors: The high expression rates in normal fallopian tube tissue,SC,SBT,LGSC,HGSC were 0.0%(0/30),3.6%(1/28),0.0 %(0/26),6.9%(2/29),76.6%(62/81).The expression differences of SF3B3 in the groups of normal fallopian tube tissues and SBT,LGSC,HGSC,the group of SC and LGSC,and the groups of SC / SBT / LGSC and HGSC were statistically significant(all P < 0.05),while in normal fallopian tube tissues and SC,SC and SBT,SBT and LGSC were not statistically significant(all P > 0.05).4.The expression of CDK7,SRp20 and SF3B3 in different types of ovarian cancer: The high expression rates of CDK7,SRp20 and SF3B3 in OSC were 56.3%(62/110),55.4%(61/110)and 58.2%(64/110);in OCCC were 37.1%(10/27),18.5%(5/27)and 33.3%(9/27);in OEC were 30.4%(7/23),17.4%(4/23)and 34.8%(8/23),respectively.The expression differences of CDK7 in OSC and OCCC(P = 0.037),SRp20 in OSC and OCCC(P < 0.001),OSC and OEC(P = 0.001),and SF3B3 in OSC and OCCC(P = 0.032)showed statistically significant,while the remaining groups were not yet statistically significant.5.The association of CDK7 with SRp20 and SF3B3: In OSC,the expression of CDK7 was associated with SRp20 and SF3B3 respectively(both P < 0.05),and the association coefficients were 0.394 and 0.521.6.The relationship between the expression of CDK7,SRp20,SF3B3 and the clinicopathological characteristics of OSC: The high expression levels of CDK7,SRp20 and SF3B3 often indicated higher tumor grade and the tendency of progression within 3 years after operation(P < 0.05).Among them,the high expression of CDK7 and SF3B3 also suggested higher clinical stage,while the expression of SRp20 had no relation with clinical stage.The expression of SRp20 was related to the patients' age(P = 0.016),the expression of SF3B3 was related to the metastasis during operation(P = 0.035).The expression levels of CDK7,SRp20 and SF3B3 were not associated with tumor diameter(P > 0.05).7.Survival analysis: pathological grade of the tumor,tumor stage,the expression of CDK7,SRp20 and SF3B3 were independent prognostic factors(P < 0.05),but the influence of patients' age and tumor diameter on prognosis were not statistical significance(P > 0.05).Conclusion: The expression differences of CDK7,SRp20,SF3B3 in OSC and OCCC,SRp20 in OSC and OEC showed statistically significant(all P <0.05).In OSC,CDK7,SRp20,and SF3B3 were highly expressed in patients with higher pathological grade,and the high expression of CDK7,and SF3B3 often indicates that the tumor is in advanced clinical stage.High expression of CDK7,SRp20,SF3B3,higher pathological grade and advanced tumor stage were independent risk factors for the prognosis of OSC patients.The high expression levels of CDK7,SRp20 and SF3B3 suggests that OSC patients are prone to progress within 3 years after operation.CDK7 may confer the ability of ovarian cancer cells to proliferate,drug resist,invade and metastasize by regulating the expression of SRp20 and SF3B3. |
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