Effect And Mechanism Of Shen-Qi Pills On Apoptosis Of Renal Cells In Mice With Ischemia Reperfusion Injury

Objective:To investigate the protective effect and mechanism of Shen-Qi pills on acute renal injury induced by I/R in mice.Methods:The total of 40 healthy male C57BL6 mice were randomly divided into 4 groups:(1)sham group;(2)renal ischemia reperfusiongroup(renal I/R group);(3)low dose group of Shen-Qi pills;(4)high dose group of Shen-Qi pills.The low dose group of Shen-Qi pills were administered 10.5 mg/kg/d of Shen-Qi pills,while the high dose group were gavaged by 21.0 mg/kg/d,for two weeks.The sham and I/R groups were administered the same amount of normal saline every day.After 2 weeks,the model of renal I/R injury was duplicated.The 4 groups were sacrificed after 24 h.Then Scr,BUN,the content of MDA and activity of SOD weredetected.HE and TUNEL staining were used to detect renal injury and apoptosis level.Andthe expression levels of bcl-2 and caspase-3 were detected by PCR and Immunohistochemistry.The expression levels of PI3 K and AKT were detected by Western blot.Results:HE and TUNEL staining showed that the Shen-Qi pills could inhibit cell damageand apoptosis.Compared with the sham group,Scr,BUN,MDA and caspase-3 levelsinthe renal I/R group were all increased(P<0.05),and SOD activity,bcl-2 level,PI3 Kand AKT protein expression level were significantly decreased(P<0.05).Compared with therenal I/R group,Scr,BUN,MDA and caspase-3 level in the Shen-Qi pills group(low dose group and high dose group)decreased(P<0.05),while SOD activity,bcl-2 level,PI3 K and AKT protein expression level were increased(P<0.05).Compared with thelow-dose group,the content of Scr,BUN and MDA and the level of caspase-3 in the high-dose group were all decreased(P<0.05),while the SOD activity,bcl-2 level,PI3 K and AKT protein expression level were all increased(P<0.05).Conclusion:The Shen-Qi pills can protect mice from I/R induced by acute renal injury by activating the PI3K/AKT signaling pathway.