The Transmission Blocking Effect Analysis Of Anti-Plasmodium Yoelii Protein Phosphatase 6 Serum

Objective: Malaria,as an infectious parasitic disease,has always threatened human health.As early as 2010,our country proposed to achieve the ambitious national goal of eliminating malaria by 2020.However,due to the increase of drug resistance and other problems,malaria treatment effectiveness decreased.To address this problem,countries are gradually focusing on combating malaria transmission.The study found that reversible protein phosphorylation regulated by kinase and phosphatase controls many cellular processes.Although the essential function of plasmodium kinase has been reported,the role of most protein phosphatase(pps)in the development of plasmodium remains unclear.Therefore,this paper mainly analyzes whether PyPPM6 has the effect of blocking transmission.Methods: The functional domain(359-644 aa)of PyPPM6 was amplified by PCR and cloned into pET32a(+)vector.PyPPM6 recombinant protein(rPyPPM6)expression was induced by IPTG,purified,mice immunization,and anti-PyPPM6 serum was collected.The anti-PyPPM6 serum titer and specificity was detected by ELISA and Western blot.The inhibitory effect of anti-PyPPM6 sera on exflagellation,ookinete formation and conversion,oocysts formation was investigated.Results: The recombinant PyPPM6 protein was successfully expressed,with an antibody titer around 1:64000.Compared with the control group,the exflagellation was significantly reduced on anti-PyPPM6 sera treatment.Ookinete formation and conversion rate decreased in anti-PyPPM6 sera treatment group.And oocysts formation was inhibited significantly in anti-PyPPM6 sera treatment group at 1:5 dilution.Conclusion: PyPPM6 protein has good immunogenicity.The anti-PyPPM6 immune serum had obvious transmission blocking activity.