Association Between CLEC4E Gene Polymorphism Of MINCLE And Pulmonary Tuberculosis Infection In A Northern Chinese Population

Background: Pulmonary tuberculosis caused by an intracellular pathogen,Mycobacterium tuberculosis continues to exist as a hazardous disease to human life globally.Genetic polymorphisms regulate resistance and susceptibility to tuberculosis.The C-type lectin receptor of family 4 member E(CLEC4E)confers protection against tuberculosis in laboratory animals but its function in influencing exposure or resistance to pulmonary tuberculosis(PTB)in humans remains obscure.Aim: We conducted this research to analyse the effects or concomitance of CLEC4 E gene variations with susceptibility to pulmonary tuberculosis in a northern Chinese population.Method: Study population: In this study,202 participants with pulmonary tuberculosis and 214 healthy controls without PTB were enrolled.Two single nucleotide polymorphisms(SNPs)rs10841845 and rs10841847 for CLEC4 E on chromosome 12 were selected with a minor allele frequency of > 0.05.DNA extraction: The Deoxyribonucleic acid was extracted from whole blood using QIAamp genomic DNA micro kit.Clec4 e gene SNP detection: PCR with high resolution melting(HRM)analysis was used for genotyping of DNA study samples and results were analysed using a Light Cycler Z480 System.Statistical analysis: SPSS version 20,IBM was used for statistical analysis,A two-tailed P value less than 0.05 was considered statistically significant.Results: We estimated and compared two SNPs,rs10841845 and rs10841847.Based on our study findings,CLEC4 E rs10841845 is protective toward pulmonary TB in dominant(AG +GG vs.AA),P< 0.001,OR = 0.485,95% confidence interval(CI)= 0.326–0.723,recessive(GG vs.AA+AG),P< 0.001 OR= 0.310,95% CI= 0.166–0.577 and co-dominant(GG vs.AA),P< 0.01,OR = 0.235,95% CI= 0.121–0.456 and(AG vs.AA),P = 0.015,OR = 0.594,95% CI = 0.391–0.904,models of genetic inheritance.Rs10841847 genotypes,both AG heterozygous(P = 0.002,OR = 0.534,95% CI= 0.356-0.080)and homozygous mutant AA(P = 0.004,OR = 0.279,95% CI = 0.112–0.069)in co-dominant model showed a significant statistical increase in the control compared to the pulmonary TB group.Recessive(P = 0.023,OR = 0.368,95%CI= 0.151–0.896)and dominant models(P< 0.001,OR = 0.494,95% CI = 0.334–0.730)both showed a statistical difference between patients and controls suggestive of providing resistance toward pulmonary TB development.Rs10841845 allele G(P< 0.001,OR = 0.516,95% CI = 0.387–0.687)and rs10841847 allele A(P< 0.001,OR = 0.556,95% CI = 0.407–0.760)were associated with protection against the development of PTB.Conclusion: Our findings suggest that variations at rs10841845 and rs10841847 of CLEC4 E genes are associated with increased individual protection against PTB development.