Combination Of Dentin Matrix Protein 1 And Bone Morphogenetic Protein 1 Promotes Bone Formation In Maxillary Sinus

Objective To investigate the role of bone morphogenetic protein 1 in dentin matrix protein 1 in promoting the differentiation of Beagle dog bone marrow mesenchymal stem cells into osteoblasts in vitro,and to detect dentin matrix protein 1 carried by Beagle dog animal experimental models.Bone formation efficiency of Beagle dog bone marrow mesenchymal stem cells combined with bone morphogenetic protein 1 target gene in the maxillary sinus region of Beagle dogs.Methods 12 equal-age 10 Kg Beagle dogs were prepared at the Experimental Animal Center of Anhui Medical University.Bone marrow of was extracted from canine sacrum in vitro,and canine bone marrow mesenchymal stem cells were separated and cultured by density gradient centrifugation.The surface antigens of canine bone marrow mesenchymal stem cells(CD44 and CD90)were identified by flow cytometry.Can be used alone or in combination with dentin matrix protein 1 and bone morphogenetic protein 1 in canine bone marrow mesenchymal stem cells.Canine bone marrow mesenchymal can be induced by dentin matrix protein 1 and bone morphogenetic protein 1 in the experiment by detecting alkaline phosphatase activity The ability of stem cells to differentiate into osteoblasts.The kit-8 cell counting kit was used to detect the effect of experimental intervention on cell proliferation.A lentiviral vector carrying the target genes of dentin matrix protein 1 and bone morphogenetic protein 1 was constructed,which respectively carried a cherry fluorescent gene fragment and a green fluorescent gene fragment.Canine bone marrow mesenchymal stem cells were transfected with a lentiviral vector carrying dentin matrix protein 1 and bone morphogenetic protein 1 target genes in vitro.The transfection efficiency was detected by observing the number of fluorescence in an upright fluorescence microscope.Get the best infection efficiency when the intensity is the strongest.Real-time quantitative PCR and Western blot were used to detect the expression of target genes and the expression of osteogenesis-related genes and proteins.Canine bone marrow mesenchymal stem cells carrying the dentin matrix protein 1 and bone morphogenetic protein 1 genes alone or at the same time were implanted on the surface of the bone replacement material Bio-Oss,and an equivalent amount of bone replacement material was used in the canine maxillary sinus lift operation Implanted into the maxillary sinus area and implanted at the same time.Immediately after surgery,the ISQ value of the implant was measured,and samples with similar ISQ values were controlled and retained,and experimental samples with large differences were discarded.M1 was removed before 3 months.Three months after surgery,the osteogenic effects of different experimental groups were compared and evaluated by measuring ISQ values,CT scanning parameters,and section staining.Results In vitro experiments,canine bone marrow mesenchymal stem cell cell surface antigens CD44 and CD90 were highly expressed,and the cell growth morphology was consistent with the characteristics of stem cells.Simultaneous administration of 5 ?g / ml recombinant bone morphogenetic protein 1 and 4 ?g / ml recombinant dentin matrix protein 1 can significantly increase the alkaline phosphatase activity of canine bone marrow mesenchymal stem cells without significantly affecting cell proliferation.Canine bone marrow mesenchymal stem cells carrying dentin matrix protein 1 and bone morphogenetic protein 1 target genes constructed by genetically engineered lentiviral vectors.The target genes were overexpressed and expressed by PCR.Protein expression increased significantly.In the Beagle animal experimental model,the target genes of dentin matrix protein 1 and bone morphogenetic protein 1 showed better implant stability and stronger osteogenesis in the maxillary sinus area than the genome carrying only dentin matrix protein 1.Compared with the dentin matrix protein 1 genome only,the target genome of dentin matrix protein 1 and bone morphogenetic protein 1 has better changes in the ISQ value of the implant.CT images show continuous high-density images surrounding the implant.The trabecular bone structure was clear and densely arranged.Conclusion The experimental group carrying the dentin matrix protein 1 and bone morphogenetic protein 1 target genes showed better osteogenesis and implant stability than the experimental group carrying only the dentin matrix protein 1 gene.Bone morphogenetic protein 1 plays a significant role in promoting bone differentiation of canine bone marrow mesenchymal stem cells induced by dentin matrix protein 1.