| Objectives:In the coronary slow flow phenomenon(CSFP)group and the normal coronary flow(NCF)group,the levels of leptin(LEP),leptin receptor(LEPR)and related clinical indicators were measured.in order to seek the risk factors about CSFP.Meanwhile,the gene and genotype distribution frequencies of LEP rs7799039,LEPR rs 1137100 and LEPR rs 1137101 were compared,to study whether the gene polymorphisms be related to the CSFP,and to find a new way to predict the occurrence of CSFP.Methods:Patients with chest pain were selected in the cardiovascular medicine department in the second affiliated hospital of Kunming medical university from September 2018 to November 2019.And all patients were operated on the coronary arteriography.According to the degree of coronary artery stenosis and the thrombolysis in myocardial infarction(TIMT)flow grade,all cases were divided into the CSFP group(n=54)and the NCF group(n=46).Besides,their clinical datas were collected,such as the fasting blood glucose(FBG)and the fasting insulin level(FINS).Furthermore,the insulin resistance was assessed by Homeostasis model in insulin resistance index(HOMA-IR),and the serum concentrations of leptin and leptin receptor were tested by enzyme linked immunosorbent assay(ELISA).As well,the gene distribution frequencies of LEP rs7799039,LEPR rs 1137100 and LEPR rs 1137101 were analyzed by the SNaPShot method,to study the correlation between the gene polymorphism and CSFP.At last,the related risk factors of CSFP were analyzed by multivariate logistic regression.Results:1.In the comparison of clinical datas,the CSFP group had higher leptin level(P=0.000)and insulin resistance index(P=0.046)than the NCF group.However,there was no significant statistical difference in the residual indexes(P>0.05).2.The SNaPShot method was used to detect the three SNP sites,and the gene distribution of study subjects was in line with the Hardy-Weinberg balance.However,there was no significant differences in the gene and genotype distribution frequencies between two groups(P>0.05).3.Further analysis of clinical indicators in the three SNPs was studied.At LEPR rs1137100,the body mass index(BMI)of GG carriers was higher than that of GA+AA carriers(P=0.044).Compared with GA carriers at LEPR rs1137101,GG carriers had higher BMI(P=0.038)and the mean platelet volume(MP V)(P=0.040).4.Binary logistic regression analysis showed that the genotypes in three SNPs had a similar risk of the CSFP.with no significant statistical differences.Finally.the result showed that the OR values of LEP and HOMA-IR were greater than one,indicating that LEP(OR=1.273,95%CI:1.026-1.579)and HOMA-IR(OR=1.096,95%CI:1.005-1.205)were the risk factors for the CSFP.Conclusions:1.LEP and insulin resistance were related to the occurrence of CSFP.Besides.LEP and insulin resistance were the risk factors for the development of CSFP.2.There was no correlation between the CSFP and clinical datas,such as sex,smoking,blood lipid level and hypertension.3.The genotype distributions of the three SNPs all conformed to the Hardy-Weinberg balance,which proved that the study subjects were representative of the population.However,the gene polymorphisms had no significant correlation with CSFP.4.LEPR rs1137100 and LEPR rs1137101 were related to BMI.The GG carriers had a higher BMI.It could be speculated that LEPR rs1137100 and LEPR rs1137101 may affect the development of cardiovascular diseases through the fat metabolism pathway.5.LEPR rs1137101 was related to MPV,and the GG carriers had a higher MPV,suggesting that LEPR rs1137101 may have a potential effect on platelet activation. |
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