Expression Of Hsa_circ₀005579 In Lung Cancer And Its Biobehavior Effects On Lung Cancer Cell Lines H292 And H460

Objective:Circular RNA?circRNA?is a kind of noncoding RNA,which has the covalently closed circular structure.According to the different formation mechanism,it has 3 types:exonic circRNA?ecircRNA?,circular intronic RNA?ciRNA?and exonic-intronic circRNA?EIciRNA?.Recently,an ocean of studies have found that circRNA is involved in multiple steps of tumor development.Lung cancer is one of the most common malignancies in the world and its increasing morbidity and mortality seriously endanger the human life and health.In recent years,increasing research about circRNA function in lung cancer shows that It will be expected to become a new target on lung cancer diagnosis and treatment.We used circRNA chip to find 3 circRNAs which express different in 3 pairs of tissues?1 pair include a patient's cancerous tissues and adjacent tissues?.We used hsa_circ₀005579 to do subsequent tumor cell?H292 and H460?function experiments,including:proliferation assay,invasion assay,migration assy,cell cycle and cell apoptosis.And we detected whether the different expressions of hsa_circ₀005579 can lead to different cell function.Methods:?1?Find 3 circRNAs which express obviously different by using circRNA chip.?2?Desigh the hsa_circ₀005579 plasmid.?3?H292 and H460 cell lines were transfected with hsa_circ₀005579 plasmid,while the control group was transfected with pcDNA3.1 plasmid.?4?The hsa_circ₀005 5 79 expression level in hsa_circ₀005579 plasmid transfected H292 and H460 cells were verified by qRT-PCR.?4?Do tumor cell function experiments:proliferation assay,invasion assay,migration assy,cell cycle and cell apoptosis.Results:1.Three circRNAs were found with significant difference of expression by using circRNA chip:hsa circ 0005579,hsa circ 0073716 and hsa_circ 0089972.These circRNAs' expression in lung cancer tissues were lower than that in paracancer tissues.2.We found the highest expression point of hsa circ₀005579 after transfecting plasmids in cell lines:H292 was 48h and H460 was 24h.3.According to the tumor cell function experiments,we found over-expression of hsa_circ₀005579 effectively inhibited proliferation,invasion as well as migration and promoting apoptosis.Besides that,It could also inhibit cell cycle progress and induce apoptosis.Conclusions:Over-expression of hsa_circ 0005579 effectively inhibits H292 and H460 cells'proliferation,invasion,migration.It can inhibit cell cycle and induce apoptosis.