Effects Of Long Non-coding RNA FEZF1-AS1 And Its Mechanism On Proliferation And Invasion Of Gastric Cancer

Objective:To investigate the effects of FEZF1-AS 1 and its specific regulatory mechanism on proliferation and invasion of GC cells SGC-7901.Materials and Methods:The cells were classified into two groups:FEZF1-AS1 small interfering RNA(si-FEZF1-AS1)and the Negative control siRNA(si-NC).Constructing FEZF1-AS1 small interfering RNA(siRNA)and negative control siRNA,then the siRNAs were transfected into SGC-7901 cells respectively.FEZF1-AS1 was knockdown in si-FEZF1-AS1 group by siRNA.The proliferative activity of GC cells was determined by colony formation assay and cell counting kit-8(CCK-8)assay,and the invasion ability was studied by wound healing assay and transwell assay.The expression levels of EMT-related molecules E-cadherin,Vimentin and Wnt pathway key gene(3-catenin was detected by RT-PCR and Western blotting.Results:1.The results of colony formation assay showed that the colony-forming capacity was impaired in si-FEZF1-AS1 group compared to the si-NC group;CCK-8 revealed that the cell proliferation of GC cells was dramatically inhibited in si-FEZF1-AS1 group.The above results showed that the proliferation ability of SGC-7901 cells in si-FEZF1-AS1 group was significantly decreased.2.Scratch tests showed that,48h after scratches in GC cells,the space between scratches was significantly wider than in si-NC group;The results of Transwell assay also indicated that the number of metastatic cells in the si-FEZF1-AS1 group was significantly decreased compared to the si-NC group.The above results showed that the migration and invasion ability of SGC-7901 cells in si-FEZF1-AS1 group was significantly decreased.3.The results of RT-PCR showed that the relative mRNA expression levels of E-cadherin were increased in si-FEZF1-AS1 group,and the relative mRNA expression levels of Vimentin was decreased.The results of western blot revealed that the expression levels of E-cadherin in si-FEZF1-AS1 group were significantly increased compared with the si-NC group,and the expression of Vimentin in the si-FEZF1-AS1 group was significantly decreased compared with the si-NC group.The above results indicated that the EMT process of SGC-7901 cells in the si-FEZF1-AS1 group was inhibited.4.The results of RT-PCR showed that the relative mRNA expression levels of ?-catenin was decreased.And the results of western blot revealed that the expression levels of ?-catenin in the si-FEZF1-AS1 group was significantly decreased compared with the si-NC group.The above results indicated that the Wnt/?-catenin signal pathway of SGC-7901 cells in the si-FEZF1-AS1 group was inhibited.Conclusion:Dysregulation of FEZF1-AS1 can suppress proliferation and invasion of SGC-7901 cells,which may be related to the Wnt/?-catenin signaling pathway.