Expression And Clinical Significance Of ACSL6 In Colorectal Cancer

Objective:Colorectal cancer(CRC)is the most common cancer in the world and the third leading cause of cancer-related deaths.CRC is a type of cancer that requires energy(ATP)and cellular components to maintain uncontrolled cell proliferation.Recent studies have attracted more and more attention in abnormal lipid metabolism to the occurrence and development of CRC.Studies have shown that ACSL6 can promote lipid anabolism in the brain and skeletal muscle to produce phospholipid and other substances to participate in the formation of cell membrane skeleton.In the process of cancer proliferation,a large number of cell membrane skeleton components are needed to supply,which provides us with the possibility to study whether ACSL6 can promote the development of tumors.In addition,studies have proved that ACSL6 can promote the production of reactive oxygen species(H2O2)in the oxidative phosphorylation process of skeletal muscle mitochondria.The oxidative damage of genes caused by the production of reactive oxygen species and subsequent mutation is one of the mechanisms of cancer,which provides us with the possibility that ACSL6 may cause cancer.Studies have found that the high-fat diet of obese people tends to lead to abnormal expression of lipid metabolism-related enzymes,such as increased expression and activity of fatty acid synthase(FAS),which is one of the pathogenesis of CRC.It is intended to find out the molecular markers by studying the expression and clinical significance of the lipid metabolism-related enzyme,ACSL6,in CRC for providing a scientific basis for the early screening of CRC and a more scientific treatment plan for CRC patients.Methods:1.Bioinformatics analysis of the expression difference of ACSL6 in colorectal cancer and normal colorectal mucosal tissues and its clinical relevance in terms of the degree of malignancy,lymph node metastasis and clinical stage of colorectal cancer;2.Collected samples of patients to analyze the expression differences of ACSL6 in colorectal cancer and normal colorectal mucosal tissues and the clinical relevance of ACSL6 in terms of the degree of malignancy,lymph node metastasis and clinical stage of colorectal cancer;3.To analyze the difference in the expression of ACSL6 in colorectal cancer cells and normal colorectal mucosal cells;Results:1.Preliminary conclusions can be drawn from bioinformatics analysis:ACSL6 is highly expressed in CRC and has relevance for clinical staging and lymph node metastasis;2.ACSL6 is highly expressed in CRC samples compared with normal colorectal mucosal tissues,and the expression of ACSL6 is correlated with the clinical stage of patients.3.ACSL6 was highly expressed in CRC cells in vitro compared with normal colorectal mucosal cells.Conclusion:ACSL6 was highly expressed in CRC tissues compared with normal colorectal mucosa tissues,and the expression of ACSL6 was correlated with clinical staging and lymph node metastasis(P<0.05).ACSL6 plays an important role in promoting the occurrence and development of colorectal cancer,and can be used as a potential biomarker for the prognosis of CRC patients.