Expression Of UCP2 In Colorectal Cancer And Its Effect On Invasion And Migration Ability

Colorectal cancer is a relatively common malignant tumor of the digestive tract that threatens human life and health.Its incidence is higher than other tumors,which is prone to metastasis and invade other organs of the body,resulting in higher mortality.Although the mechanism of the development of colorectal cancer has not yet been clearly studied,according to our existing research,mitochondrial uncoupling protein 2(uncoupling protein-2,UCP2)can affect colorectal by regulating the ability of metabolism The occurrence and development of cancer.UCP2 is a mitochondrial inner membrane protein that has the ability to inhibit the generation of reactive oxygen species,resist apoptosis,maintain mitochondrial membrane stability,and can affect macrophage-mediated immune responses and inflammatory processes.UCP2 is closely related to the ability to regulate the proliferation and apoptosis of tumor cells.By adjusting the microenvironment of tumors,it creates a condition that is conducive to the unlimited growth of tumor cells and promotes the development of tumors.There is research evidence that the expression of UCP2 may affect the invasion and migration of tumor cells.Some studies have found that UCP2 may play a key role in the development of colorectal cancer.Colorectal cancer generally has no obvious symptoms at an early stage,it is easy to be ignored by the patient,and after the symptoms appear,the tumor has developed to an advanced stage,so the early diagnosis of colorectal cancer can improve the patient's prognosis and survival time.UCP2 may be an effective target for colorectal cancer,which is of great significance for early diagnosis and treatment.Our research focuses on analyzing the correlation between UCP2 expression and the prognosis of colorectal cancer patients,and in vitro cell experiments to study the effect of UCP2 expression on the invasion and migration of colorectal cancer cells.ObjectiveExplore the expression of UCP2 in colorectal cancer and adjacent tissues;Analyze the correlation between UCP2 expression and pathological parameters and prognosis of colorectal cancer patients;study the effect of silencing UCP2 expression on the invasion and migration ability of colorectal cancer cells.MethodsImmunohistochemical methods were used to detect the expression of UCP2 in 100 cases of colorectal cancer patients and 18 cases of adjacent tissue samples.Chi-square test was used to analyze thecorrelation between UCP2 and clinicopathological parameters,Kaplan-Meier was used to draw survival curves,and Cox proportional hazards model was used to perform univariate and multivariate analysis of overall survival and relapse-free survival.UCP2 in colorectal Impact of cancer prognosis.RT-PCR and Western blot were used to detect the expression level of UCP2 in 6 colorectal cancer cell lines,and two significantly high expression cell lines(CACO2 and SW1116)were selected for subsequent cell function experiments;using Si RNA transfection technology,Down-regulate the expression of UCP2 in colorectal cancer cell lines,and further verify the down-regulation efficiency of UCP2 by RT-PCR and Western blot.Transwell matrigel test and scratch test were used to detect the effect of silencing UCP2 on the invasion and migration of colorectal cancer cells.Results1.Immunohistochemical results indicated that the positive and negative expression rates of UCP2 in colorectal cancer tissue were 72%(72/100)and 28%(28/100),respectively,and the difference was statistically significant(P <0.05).2.Analysis of clinicopathological parameters revealed that high expression of UCP2 was significantly correlated with tumor T stage,N stage,M stage,and TNM stage(P <0.05).Survival analysis found that the overall survival time and recurrence-free survival time of patients with colorectal cancer with high expression of UCP2 were significantly shorter than those with low expression(P <0.05).COX regression analysis found that the expression of UCP2 is an independent risk factor affecting the prognosis of colorectal cancer patients.3.The results of Transwell Cell Matrigel Experiment and Scratch Experiment showed that the ability of colorectal cancer cells to inhibit the invasion and migration of colorectal cancer cells can be significantly inhibited after down-regulating the expression of UCP2.Conclusions1.The expression of UCP2 is significantly up-regulated in colorectal cancer tissue,and its expression level is closely related to the depth of primary tumor invasion,the number of positive lymph node areas,the presence of distant metastasis,and TNM staging.UCP2 expression is an independent risk factor that affects the prognosis of colorectal cancer patients.2.Silencing UCP2 can significantly inhibit the invasion and migration of colorectal cancer cells.UCP2 plays an important role in the development of colorectal cancer.