Effcct Of Single Nucleotidepolymorphism On The Analgesic Effect Of Sufentanil

Objective:To study the effect of genetic mutation site polymorphism on postoperative analgesia of sufentanil.Methods:A total of 224 patients who underwent hysterectomy from August 2018 to October 2019were enrolled.All subjects were divided into routine and study groups according to the random number table method.example.Firstly,the relationship between postoperative analgesic sufentanil consumption and preoperative pain threshold and pain threshold was studied.The index included VAS score,sufentanil consumption and Ramsay score in the two groups.Secondly,the ORRM1 A118G gene was explored.The effect of polymorphism on pain threshold,pain threshold and analgesic effect,the index includes allele frequency and analgesic effect;finally,the effect of CYP3A on its enzymatic activity and analgesic effect was studied.The index was the same as that of experiment 2.It also includes allele frequencies as well as analgesic effects.ResuLts:(1)Partl?:The Bromage grade and VAS of the study group were significantly lower than the conventional group.The difference between the two groups was significant,P<0.05,the difference was statistically significant.?The study group not only consumed the average the amount was significantly lower than the conventional group,and the average consumption ratio of sufentanil to body weight was significantly lower than that of the conventional group,P<0.05,the difference was statistically significant;?the score of the adverse reaction immediately after the conventional group and the first the adverse reaction scores of the second 24h were higher than the study group.The difference between the two groups was significant,P<0.05,and the difference was statistically significant.(2)Part2:?The distribution of wild-type homozygotes,heterozygotes and mutant homozygotes of OPPM1 A118G allele was consistent with Hardy-Weinberg equilibrium,but P>0.05,the difference was not statistically significant;?Comparative three groups the pain threshold between patients was not significant,P>0.05,the difference was not statistically significant;?compared the pain tolerance threshold between the three groups,A/A group was significantly higher than A/G group,A/G group was significantly higher than G/G group,the difference was significant,P<0.05,the difference was statistically significant;?A/A,A/G and G/G group immediately after surgery and 24h postoperative mean VAS score The difference was not statistically significant(P>0.05);?the difference between A/A,A/G and G/G in the 24h sufentanil consumption after surgery was significant,G/G group was significantly higher than A/G group and A/A group,P<0.05,the difference was statistically significant;?A/G and A/A group in the first 24h sufentanil consumption after operation The difference was not obvious,P>0.05,the difference was not statistically significant;?the 24h postoperative sufentanil consumption was positive with the OPPM1 A118G allele number.(r=0.20),and the difference between the groups was significant,P<0.05,the difference was statistically significant;?There was not significant difference in the incidence of adverse reactions between nausea and vomiting and other sufentanil in the groups.P>0.05,the difference was not statistically significant.(3)Part3?The distribution of wild-type homozygotes,heterozygotes and mutant homozygotes of CYP3A4*1G allele was consistent with Hardy-Weinberg equilibrium,but P>0.05,the difference was not statistically significant;?CYP3A4*1 There were no significant differences in the mean VAS scores between the CYP3A4*1/*1G and CYP3A*1G/*1G groups immediately after surgery and 24h after surgery(P>0.05).?The difference between the CYP3A4*1/*1 type,CYP3A4*1/*1G type and CYP3A4*1G/*1G type in the 24h sufentanil consumption was significant,and the CYP3A4*1G/*1G group was significant.Compared with the remaining two groups,P<0.05,the difference was statistically significant.?There was a negative correlation between the 24h sufentanil consumption and the number of CYP3A4*1G alleles after surgery(r=-0.14).The differences between the groups were significant,P<0.05,and the differences were statistically significant.Conclusion:The OPPM1 A118G polymorphism is closely related to the preoperative pain threshold,and there is a positive correlation between the sufentanil consumption and the OPPM1 A118G allele at 24h after surgery.In addition,there was a negative correlation between sufentanil consumption and the number of CYP3A*1G alleles 24 h after surgery.Therefore,genetic polymorphism can affect the consumption of sufentanil and postoperative analgesia to a certain extent.