Expression Of Interleukin(IL)-35 In The Valve Of Rheumatic Mitral Stenosis And Stimulate Effect

Objective:To compare whether there is a difference in the expression of IL-35 in patients with rheumatic mitral stenosis(RMS)and patients with mitral valve prolapse.relationship.Compare whether there are different effects on the stimulation of peripheral blood mononuclear cells(PBMC)from patients with rheumatic mitral stenosis and healthy controls under the same concentration of IL-35.To clarify the correlation between IL-35 and the degree of valve stenosis in rheumatic mitral stenosis,and initially explore the role of IL-35 in stimulating autocrine.Methods:1.Collect the diseased valve tissue of patients with rheumatic mitral stenosis mainly based on mitral stenosis,and divide them into two groups of moderate and severe mitral stenosis according to the degree of mitral stenosis and patients with mitral valve prolapse The tissues were the control group,including 10 patients with moderate mitral stenosis,12 patients with severe mitral stenosis,and 5 patients with mitral valve prolapse.Among them,all patients underwent mitral valve replacement surgery.The relative expression of IL-35 in valve tissue was determined by Western blotting.2.Collect PBMC derived from RMS patients and PBMC derived from healthy volunteers.IL-35 stimulates PBMC in vitro to obtain the optimal stimulation condition.200ng/mL IL-35 stimulates for 48h,and under this condition,stimulates PBMC from different sources,application Enzyme-linked immunosorbent assay was used to determine the expression of IL-35 in PBMC after stimulationResults:1.Collect the diseased valve tissue of patients with rheumatic mitral stenosis mainly based on mitral stenosis,and divide them into two groups of moderate and severe mitral stenosis according to the degree of mitral stenosis and patients with mitral valve prolapse The tissues were the control group,including 10 patients with moderate mitral stenosis,12 patients with severe mitral stenosis,and 5 patients with mitral valve prolapse.Among them,all patients underwent mitral valve replacement surgery.The relative expression of IL-35 in valve tissue was determined by Western blotting.2.Collect PBMC derived from RMS patients and PBMC derived from healthy volunteers.IL-35 stimulates PBMC in vitro to obtain the optimal stimulation condition.200ng/mL IL-35 stimulates for 48h,and under this condition,stimulates PBMC from different sources,application Enzyme-linked immunosorbent assay was used to determine the expression of IL-35 in PBMC after stimulation.Conclusion:1.Western blot analysis revealed that IL-35 was expressed in the RMS diseased valve.2.Compared with the prolapse control group,the expression of IL-35 in the diseased mitral valve tissue of patients with rheumatic mitral stenosis is increased,and the expression of IL-35 is positively correlated with the degree of stenosis of the valve tissue.That is,as the degree of valve stenosis worsens,the more IL-35 is expressed.Therefore,it is concluded that IL-35 participates in the process of regulating the pathological changes of rheumatic mitral valve tissue stenosis.3.In in vitro experiments,PBMC can be stimulated by IL-35 to produce more IL-35,which proves that IL-35 can promote self-synthesis and form positive feedback,which helps to play its biological role.4.Under the same stimulus conditions,PBMCs from patients with rheumatic mitral stenosis were higher than PBMCs from healthy controls by ELISA.The content of IL-35 in the cells was higher by ELISA,indicating the high sensitivity of the immune response of RMS organism.