Changes Of Serum NSE?ProGRP And CA199 In Patients With NSCLC And Their Relationship With Disease Development

Objective:In clinical practice,the detection of serum tumor markers as an auxiliary diagnosis of primary lung cancer,combined detection of tumor markers has become a research hotspot in recent years.The purpose of this study was to investigate the significance of serum NSE(Neuron specific enolase),ProGRP(Pro-gastrin-releasing peptide),CA199(Carbohydrate antigen 19-9)in the diagnosis of NSCLC and the relationship between serum NSE,ProGRP and CA199 levels and different pathological characteristics and clinical stages of NSCLC.Methods1 Research object:From January 2017 to September 2017,90 NSCLC patients(NSCLC group),52 males and 38 females,aged(57.6±11.0),were selected.90 healthy subjects were selected as the control group,48 males and 42 females,aged(56.3± 12.1)years.The serum NSE,ProGRP and CA199 levels of the two groups were detected,and the differences of serum NSE,ProGRP and CA199 levels in NSCLC patients with pleural effusion,lymph node metastasis,different TNM stages,histological differentiation degree and different pathological types were analyzed.2 Collection and treatment of specimens:In the morning after admission,3ml of venous blood was collected on an empty stomach,and NSE,ProGRP and CA199 monoclonal antibodies were added.NSE,ProGRP and CA199 in samples and standards were combined with monoclonal antibodies,and PBS was washed for 5min.According to the proportion of 1:500,add 5ml of the first antibody of sheep sign,place it overnight at 4?,wash it with PBS buffer for 3 times,5min each time,add 2ml of the second antibody from mouse source(1:1000),place it at room temperature for 2h,wash it with PBS for 5min.When the substrate is added,peroxidase will make the colorless developer appear blue,and the stop solution will turn yellow.The OD value was measured at 450 nm.All reagents are instrument matched reagents,and all operations are carried out in accordance with the instructions of the instrument and kit.3 Statistical methods:The expression of measurement data is(x ±s),and the t-test of two groups of independent samples is used for the comparison between groups;the ?2 test is used for the comparison between groups of counting data;P value<0.05 indicates that the difference is statistically significant,and the statistical software is SPSS 16.0 version.Results1 The mean and standard deviation of serum NSE in NSCLC group and healthy control group were(31.8± 13.7,7.6±3.3).The mean and standard deviation of serum ProGRP in NSCLC group and healthy control group were(938.6±573.1,16.4±5.6).The mean and standard deviation of serum CA199 in NSCLC group and healthy control group were(59.6± 15.2,14.2±6.5).The levels of NSE,ProGRP and CA199 in serum were higher than those in healthy control group(P<0.05);2 The serum levels of NSE,ProGRP and CA199 in stage ?+? NSCLC patients were(27.5± 12.5,775.2±442.1,45.4± 11.2).The serum levels of NSE,ProGRP and CA199 in stage ?+? NSCLC patients were(38.2±11.4,1168.8±553.6,67.8±14.3).The levels of NSE,ProGRP and CA199 in patients with stage ?+? NSCLC were significantly higher than those in patients with stage ?+?(P<0.05);3 The serum levels of NSE,ProGRP and CA199 in NSCLC patients with lymph node metastasis were(35.0 ± 13.2,1109.2±555.8,66.5± 15.8),and those in NSCLC patients without lymph node metastasis were(26.2± 12.8,740.4±499.6,49.2± 12.4).The levels of NSE,ProGRP and CA199 in NSCLC patients with lymph node metastasis were significantly higher than those in NSCLC patients without lymph node metastasis(P<0.05);4 The serum levels of NSE,ProGRP and CA199 in NSCLC patients with high moderate differentiation were(30.6 ± 12.8,898.9± 559.7,53.8± 14.1),and those in NSCLC patients with low differentiation were(34.2± 12.6,1044.2±565.4,56.6± 17.2).There was no significant difference in serum NSE,ProGRP and CA199 levels among NSCLC patients with different degrees of differentiation(P>0.05);5 The serum levels of NSE,ProGRP and CA199 in NSCLC patients with pleural effusion were(33.2±13.2,994.0±566.5,53.2± 14.2).The serum levels of NSE,ProGRP and CA199 in NSCLC patients without pleural effusion were(31.3± 13.5,925.4±565.4,52.0± 16.1).There was no significant difference in serum NSE,ProGRP and CA199 levels between NSCLC patients with or without pleural effusion(P>0.05);6 The serum levels of NSE,ProGRP and CA199 were(35.2±12.8,1059.0±556.2,52.8± 13.6)in adenocarcinoma and(33.3± 12.0,948.8±523.8,59.0±17.3)in squamous cell carcinoma.There was no significant difference in serum NSE,ProGRP and CA199 levels between patients with different pathological types of NSCLC(P>0.05).ConclusionSerum NSE,ProGRP and CA199 levels of NSCLC patients were significantly higher than those of healthy people.The detection of NSE,ProGRP and CA199 in peripheral blood has certain value in the differential diagnosis between NSCLC and healthy people.The expression level of NSE,ProGRP and CA199 in serum is closely related to the clinical stage of NSCLC.The later the stage is,the higher the expression level is.The expression levels of NSE,ProGRP and CA199 in serum were closely related to the lymph node metastasis in NSCLC patients,and they increased significantly in NSCLC patients with lymph node metastasis.The expression levels of NSE,ProGRP and CA199 in serum have no high clinical value for the differentiation of NSCLC cancer cells,the presence or absence of pleural effusion and the prediction of pathological types.According to the expression level of NSE,ProGRP and CA199 in serum,the lymph node metastasis and clinical stage of NSCLC patients can be preliminarily determined.