Correlation Study Between The Genesis And Progress Of Coronary Atherosclerotic Heart Disease With Younger Patients And ANP(T-2238C)Gene

Objective:Coronary Atherosclerotic Heart Disease(CAHD),a menace to human health and life,has soaring morbidity rate and mortality rate in China.In recent years,an increasing number of younger people have suffered from this disease.CAHD is a complicated disease with diverse pathogenic factors.Many causes of CAHD have been found,but they can hardly explain all CAHD symptoms.The rapidly developing molecular epidemiology,molecular genetics and molecular biology have contributed to our better understanding of the role that the gene plays in CAHD.Some researches revealed that the resequencing of the 2238 site of Atrial Natriuretic Peptide(ANP)precursor gene would alter the translation of two Arginine in ANP.This change would cause the mutant ANP to contain the endotheliocyte proliferation and angiogenesis by increasing active oxygen,which is closely associated with CAHD.Therefore,this study aims at discussing correlation between the increasing CAHD morbidity among younger patients and ANP(T-2238C)gene polymorphisms,in a bid to test if there is an association between mutant gene locus and CAHD.This study could provide the theory for screening the young and middle-aged for CAHD gene,improve the primary prevention of CAHD,cut the morbidity rate of CAHD among younger patients and acute myocardial infarction(AMI),and advance the well-being of the people.On top of that,the study result can guide high-risk patients to take preventive medication,which can provide the theory for the forward-looking researches on evaluating gene drug efficacy.Methods:In this study,we took the patients(no more than 50 years old before the examination)referred for coronary angiography by Qilu Hospital of Shan Dong University as the target sample,set up selection criteria,figured out the target group and followed up the patients;patients with CAHD were tagged as the experimental group(they were separated into CABG experimental group and PCI experimental group)and patients without CAHD were tagged as the control group,baseline clinical profile that contained several conventional dangers was made;in the collection of patients'blood,we used midi kit(Beijing Biotech,Beijing,China)to extract whole-blood genome under the guidance of the manufacturer's instruction;the purity and density of all DNA samples were examined;we genotyped ANP(T2238C)with TaqMan SNP genotyping kit(Applied Biosystems,Foster City,CA,USA)and SNP gene analysis.Then,we put the data into excel2016 and did statistical analysis with SPSS 25.0(IBM,Armonk,NY,USA).In the analysis,categorical variables were displayed by the number(percentage),normal-distributed continuous variables were displayed by the formula:means ± standard deviation,and abnormal-distributed categorical variables were turned into orderly categorical variables which were tested by rank sum.Besides,the each interclass were cross-checked by t test and ?2 test;the correlation between polymorphisms and the number of pathologically-changed blood vessels was assessed by Spearman's rank-correlation test and the threshold of significance was ?=0.05.Chi-square tests(?2 tests)aimed at testing if the distribution of the genotypes matched Hardy-Weinberg equilibrium(theoretical frequency);rc chi-square tests were used to compare the genotypes in different interclasses and Allele frequency AF.When p<0.05 is attained,this analysis would be meaningful statisticallyResults:The correlation between the morbidity rate of CAHD among younger people and polymorphisms(rs=0.358,p<0.001),In the experimental group,the number of culprit blood vessels are associated with polymorphisms(rs=0.551,p<0.001).When the odds ratio(OR)of TC genotype is compared with TT genotype,the OR ration was 2.874 and the 95%CI(interval)is(1.259-5.561);When the odds ratio(OR)of TC genotype is compared with CC genotype,the OR ration was 0.091 and the 95%CI(interval)is(0.633-1.881)Conclusions:Both the morbidity rate of CAHD among younger people and the number of culprit blood vessels are associated with polymorphisms and homozygous mutation of ANP(2238T/C),among which TT homozygous mutation may increase the morbidity rate of CAHD among younger people.In genetics,CAHD is caused by both environment and polygenes.Therefore,since the sample volume is small in this study,It cannot represent the characteristics of CAHD in the younger age group,there might be a type II error.And comprehensive analysis of genetic cause of CAHD still needs genome-wide association analysis with big sample volume held by more research centers in different areas.