The Expression And Role Of Periostin During Distraction Osteogenesis In Mice Bone Marrow Mesenchymal Cells

BackgroundAs a popular endogenous bone tissue engineering,the biological mechanism of distraction osteogenesis remains unexplored.The bone marrow mesenchymal cells play an important role during the distraction osteogenesis.The bone marrow mesenchymal cells are one of the most stress-sensitive response cell.The expression and role of periostin during distraction osteogenesis have not been reported.In this studythe mics bone marrow mesenchymal cells were isolated and cultured.A multiple-unit uniaxial stretching device was used to construct a cell distraction osteogenesis model in vitro.Different mechanical tensions were applied on the mice bone marrow mesenchymal cells to stimulate osteogenic differentiation.The expression of osteogenic markers and the expression of periostin were detected during the process.Further the exogenous periostin was given to investigate the role of periostin during distraction osteogenesis.Materials and Methods1.The isolation,culture and identification of the mics bone marrow mesenchymal cellsThe density gradient centrifugation was used to isolate and culture of the mics bone marrow mesenchymal cells.The differentiation capacity of the mics bone marrow mesenchymal cells were identified according to the morphology of the cells,the formation of mineralized nodules and type ? collagen.2.The expression of periostin during distraction osteogenesis in the mics bone marrow mesenchymal cellsThe multiple-unit uniaxial stretching device was applied to detect the expression of periostin and osteogenic markers in the mics bone marrow mesenchymal cells under 5%(Group A)?10%(Group B)and 15%(Group C)stretch tension3.The role of periostin during distraction osteogenesis in the mics bone marrow mesenchymal cellsThe Og/L(Group A),10g/L(Group B)and 20g/L(Group C)of the exogenous periosin were uesd to intervent the process under 15%stretch tension by the multi-unit cell stretching device.The expression of periostin and the expression of osteogenic markers in the mics bone marrow mesenchymal cells were detected.Results:1.The isolation,culture and identification of the mics bone marrow mesenchymal cellsIn this study,the density-gradient centrifugation was succeeded in isolating the mics bone marrow mesenchymal cells with high purity.The third generation of the mics bone marrow mesenchymal cells have the ability of osteogenesis and chondrogenic differentiation,which accords with the properties of bone marrow mesenchymal cells.2.The expression of periostin during distraction osteogenesis in the mics bone marrow mesenchymal cellsIn this study,the cell count,morphology and arrangement of bone marrow mesenchymal cells in three groups of mice had no significantly different(P>0.05).There was no significant difference in the expression of periostin and the expression of osteogenesis markers in the mics bone marrow mesenchymal cells in the three groups before stressing(P>0.05).The expression of periostin and the expression of osteogenesis markers in the mics bone marrow mesenchymal cells in group B were higher than those in group A and group C in 2 h and 12 h after the addition of force(P<0.05).3.The role of Periostin during distraction osteogenesis in mice bone marrow mesenchymal cellsIn this study,the expression of periostin and osteogenesis markers in the mics bone marrow mesenchymal cells in group C were stronger than those in group A and group B under 15%stretch tension.Conclusion:1.The different uniaxial stretching forces can promote the secretion of periostin in the mics bone marrow mesenchymal cells,which the dynamic distraction force of 10%of the shape variables not only promotes the ability of osteogenesis,but also promotes the secretion of periostin by bone marrow mesenchymal cells2.With the exogenous periostin,the osteogenesis ability of the bone marrow mesenchymal cells was significantly improved and 20g/L periostin can promotes osteogenesis most strongly,which indicating that periostin could promote the osteogenesis ability during the distraction osteogenesis in mics bone marrow mesenchymal cells.