Expression And Clinical Significance Of Interferon-induced Proteins With Tetratricopeptide Repeats 2 In Human Clear Cell Renal Cell Carcinoma

Objectives:Studies have shown that interferon-induced proteins with tetratricopeptide repeats 2(IFIT2)plays an important role in tumor development.The aim of our research is to investigate the expression of IFIT2 in human clear cell renal cell carcinoma(ccRCC)and to analyze its clinical significance.Methods:The immunohistochemistry assay and the tissue micro-array were used to examine the IFIT2 expression in clear renal cell carcinoma tissues as well as adjacent normal tissues.We used Wilcoxon test to analyze the difference of IFIT2 expression levels in cancer and normal tissues.We used Chi-square and Log-rank test to analyze the IFIT2 expression in cancer tissues in relation to patients' clinical parameters and prognoses respectively.And then we use Cox model to analyze correlation of different indexes and prognosis.Results:We analyzed the results of immunohistochemistry and the results showed that weak staining of IFIT2 could be found in the cytoplasm and on the membrane of the cancer cells,while positive staining of IFIT2 could be found in normal renal tissues,and the difference between renal cancer tissues and adjacent normal tissues was statistically significant(P<0.0001).The survival analysis showed that the overall survival rate of the patients with lower IFIT2 expression was significantly poorer than those with higher IFIT2 expression(HR=3.006,95%CI:1.051?8.597,P=0.040).The COX model also showed that age(HR=3.538,95%CI:1.586?7.891,P=0.002),pathological stage(HR=5.217,95%CI:2.168?12.555,P<0.001),TNM stage(HR=4.382,95%CI:1.560?12.311,P=0.005)and IFIT2 expression(HR=5.646,95%CI:1.836?17.357,P=0.003)could be used as the independent risk factor for the prognostic prediction of the patients.Moreover,the enrichment analysis based on ccRCC expression profile data from TCGA revealed that the significant terms of biological process and pathways were focused on "response to virus(GO:0009615)","T cell activation(GO:0042110)","regulation of innate immune response(GO:0045088)","cellular response to interferon-gamma(GO:0071346)","NOD-like receptor signaling pathway(hsa04621)","Chemokine signaling pathway(hsa04062)","PI3K-Akt signaling pathway(hsa04151)","Toll-like receptor signaling pathway(hsa04620)"and so on.Conclusions:Current findings suggest that the expression of IFIT2 is lower in renal cancer tissues than that in adjacent normal tissues.IFIT2 may serve as a valuable prognostic marker of the renal cancer patients,and the potential mechanism of decreased IFIT2 expression in the progression of ccRCC merits further investigation.