Bifidobacterium From Breast-fed Infant Faeces Prevent High-fat Diet Induced Mice With Glucose And Lipid Metabolism Disorders

Long-term high-fat diet is an important factor that causes metabolic syndrome,which is closely related to obesity,hyperlipidemia and impaired glucose tolerance.Due to some side effects caused by drugs and surgery,seeking to prevent metabolic syndrome which caused by high-fat diet has attracted more attention in the field of food science.Actually,Bifidobacterium is the most frequently used human probiotics because of their health-promoting properties.However,the prospects of Bifidobacterium application in metabolic diseases have not been extensively explored,and the molecular mechanisms are not fully elucidated.Therefore,the current study aims to screen Bifidobacterium strain from infant faeces and elucidate the underlying mechanisms of Bifidobacterium involving prevent metabolic syndrome,both in vitro and in vivo.The results indicated that Bifidobacterium lactis A12 was an acid-and gastrointestinal-tolerant and bile-sensitive strain,and the cell-free supernatant(CFS)of A12 showed high a-glucosidase inhibitory activity(18.57%).In addition,the CFS of A12 exhibited excellent inhibitory activity against glucose transport in transwell cell model(25.93%).On the one hand,A12 alleviated glucose intake and insulin resistance by down-regulating the mRNA abundance of Sucrase-isomaltase(S-1)and Glucose Transporter 2(GLUT-2).On the other hand,A12 prevented leptin resistance as well as restored the function in islet and liver tissues by down-regulating Sterol Regulatory Element Binding Protein-1(SREBPs),up-regulating Proglucagon(GCG)and stimulating the secretion of Glucagon-like Peptide-1(GLP-1).Moreover,A12 increased the abundance of short-chain fatty acid-producing bacteria(Faecalibaculum?Marvinbryantia?uribaculaceae?Anaeroplasma).In addition,PICRUST results indicated that A12 could regulate metabolism,genetic information processing and environment information processing related pathway.Eventually,we intend to elucidate the underlying mechanisms of Bifidobacterium with reducing body fat deposition and increasing glucose tolerance,and provide some new ideas for the prevention of metabolic syndrome.