Study On The Relationship Between Serum Neuropeptide S Level And Arousal In Patients With Obstructive Sleep Apnea

0bjective To study the relationship between serum neuropeptide S(NPS)level and arousal in patients with obstructive sleep apnea(OSA),To explore the mechanism of OSA related arousal based on NPS,so as to provide a theoretical basis for the further development of neuropeptide S-related drugs to improve daytime sleepiness caused by frequent arousals in OSA patients.Method(1)From December 2018 to June 2019,62 patients diagnosed as OSA by PSG in Huainan first people's Hospital Affiliated to Bengbu Medical College were randomly selected Results the patients were divided into mild group(18 cases),moderate group(20 cases)and severe group(24 cases),and 32 healthy volunteers were selected as the control group at the same time.(2)The general data,serum NPS level,Epworth Sleepiness Scale(ESS)score and PSG data of all subjects were collected.The judgment of arousal was in accordance with the scoring rules of sleep respiratory events issued by American Sleep Medical Association in 2007,and the serum NPS level was measured by enzyme labeled antibody method.(3)Through the analysis and comparison of general data,serum NPS level,ESS score and PSG data between OSA patients with different disease severity and the control group,as well as the differences of data between OSA groups,the significance of these data was discussed.We also analyzed the correlation between the level of serum NPS and arousal in OSA patients,as well as the correlation between the two and the data,and focused on the possible mechanism of arousal and the role of NPS in it.Result(1)There were significant differences in serum NPS level,ESS score and BMI between OSA group and control group(P< 0.01).With the aggravation of OSA,the level of serum NPS,ESS score and BMI were all increased(P < 0.05).(2)There were significant differences in AHI,MAI,non rapid eye movement sleep(N1),N2%,N3% and REM %(P< 0.05).The difference of MAI,N1%,N2% and N3% in mild group was statistically significant(P<0.05).Except for the proportion of REM %,the percentage of MAI and N1 % in severe group was higher than that in mild group and moderate group(P< 0.05).By comparing the OSA group with the control group,it was found that MAI and N1 % in OSA patients increased(P< 0.05),and N3%decreased(P< 0.05).(3)The level of serum NPS was positively correlated with MAI(r= 0.827),ESS(r=0.692),AHI(r= 0.789),N1%(r= 0.707)(all P< 0.05),but negatively correlated with N2 %(r=-0.679),N3%(r=-0.483)(all P< 0.05).(4)There was a positive correlation(P< 0.05)between the times of nocturnal arousal and ESS(r= 0.766),BMI(r= 0.549),AHI(r= 0.782),N1%(r= 0.797),and a negative correlation(P< 0.05)between the times of nocturnal arousal and N2%(r=-0.743),N3%(r=-0.617).Conclusion(1)Compared with the control group,the level of serum neuropeptide S and arousal in OSA patients were significantly increased.(2)With the aggravation of OSA,the level of serum NPS,arousal index,ESS score and the proportion of N1% increased,while the proportion of N3% decreased.It is suggested that the sleep change of OSA patients is mainly due to the increase of arousal times,which results in the disruption of normal sleep cycle,the change of sleep cycle time in each stage,and the decline of sleep quality,thus aggravating daytime sleepiness.(3)There was a positive correlation between the level of serum NPS or arousal and the proportion of BMI,AHI,N1%,N2%,N3%.(4)There is a correlation between the level of serum NPS and arousal in OSA patients.However,the sample size of this experiment is single and small,and the level of serum neuropeptide S is not monitored in real time.It can never be judged whether the release of NPS in the body before or after the occurrence of arousal in OSA patients at night.The relationship between serum NPS level and arousal in OSA patients needs further study.