| Objective: Electroacupuncture at DU26 and ST36 were used to intervene in Middle Cerebral Artery Occlusion(MACO)reperfusion model rats.The neuroethology and brain histology of rats were detected.The cerebral infarction and blood flow were observed by imaging.Improvement of rat brain microcirculation and possible mechanism of action.Method: 170 male SD rats were randomly divided into sham-operated group 34 and model 136.The modified model was used to prepare MCAO rat model by modified thread plug method.After successful modeling,it was divided into model group,electroacupuncture group and non-acupoint group.The electroacupuncture group takes acupuncture points at DU26 and ST36,and the non-acupuncture group takes bilateral nonacupuncture points under the bilateral flank,and starts intervention six hours after the operation.7 consecutive days.Rats in each group were scored by the Zea-longa method at 24 hours,3 days,and 7 days after modeling to observe the neurological deficit of rats;small animal magnetic resonance imaging(MRI)was performed at 3 hours,3 days,and 7 days.MRI to observe cerebral infarction;Perfusion weighted imaging(PWI)is used to detect Cerebral blood flow(CBF),Cerebral blood volume(CBV),Mean trasit time(MTT)and average blood flow passage time,observe the cerebral blood flow in MCAO rat cerebral infarction area after acupuncture at DU26 and ST36;on the 7th day,the brain of rat was fixed by cardiac perfusion method and HE staining and TTC staining were done Observation of histomorphology.Results: 1.Neurobehavioral scores: The behavioral scores of the three groups of rats gradually decreased on day 1,day 3,and day 7.The nerve score of the electroacupuncture group was better than that of the model group and the non-acupoint group,and the difference was statistically significant(P?0.05).There was no significant difference between the nonacupoint group and the model group(P?0.05).2.Cerebral infarction volume: In the sham-operated group,the neuron cells in the brain tissue of the rats had clear structural morphology and neatly arranged cells.The morphology and size of the cells were normal,with clear nucleoli and evenly stained nucleoplasm.Pyramidal cells have obvious protrusions,the gap between nerve cells and capillaries is normal,no cell swelling is seen,and the tissue structure is normal.In the model group and the non-acupoint group,there are large pieces of necrotic cells in the neurons of the brain tissue.The cells are arranged chaotically,with irregular shapes,such as rhombuses or triangles.Cytoplasmic edema,disintegration,necrotic area can be seen microglial hyperplasia and vascular hyperplasia,foam-like cells increased in the necrotic area;loose edema in the gap between cells and blood vessels is obvious.Electroacupuncture group also showed necrosis of neurons in brain tissue,red stained granular areas,microglial hyperplasia and vascular hyperplasia,the gap between cells and blood vessels was reduced,the cell morphology was round or oval,and the tissue morphology was compared with the model The Hefei point group was improved,the arrangement was more orderly,and the number of disintegrating cells was small.The sham-operated group had clear and symmetrical brain structure,centered ventricle,gray matter with no edema,no necrosis,no structural disorder,and no abnormal signal changes in the image.The hyperintensity of the lobe and striatum changes,and edema and swelling of brain tissue in some corresponding areas;the ventricular edema is obvious,affecting the contralateral ventricle.3.Morphological changes of brain tissue: TTC staining showed that normal brain tissue was red and the infarct area was white.Compared with the model group,the acupuncture group had an improvement,the difference was significant(P=0.000<0.05),and the nonacupoint group had no significant difference compared with the model group(P=0.086>0.05).4.Changes in cerebral blood flow: CBF: On the third day,the CBF value of the electroacupuncture group was higher than that of the model group and non-acupoint group,but the difference was not statistically significant(P=0.452>0.05,P=0.564>0.05,).On the seventh day,there was no significant difference in the CBF value between the electroacupuncture group and the sham operation group(P=0.114>0.05);the CBF value of the electroacupuncture group was higher than the model group,and the difference was statistically significant(P=0.000<0.05);The CBF value of the electroacupuncture group was higher than that of the non-acupoint group,and the difference was statistically significant(P=0.000<0.05).Compared with the same group at different times on the third day and the seventh day,compared with the seventh day in the electroacupuncture group,the CBF value on the seventh day was higher than the third day,the difference was statistically significant(P=0.000<0.05);Compared with the seventh day of the sham operation,model group,and non-acupoint group,there was no significant difference in CBF value(P=0.618>0.05,P=0.512>0.05,P=0.155>0.05).CBV: On the third day,the CBV value of the electroacupuncture group was higher than that of the model group and non-acupoint group,and the difference was statistically significant(P=0.012<0.05,P=0.004<0.05,).On the seventh day,the CBV value of the electroacupuncture group was higher than that of the model group and non-acupoint group,and the difference was statistically significant(P=0.000<0.05).Comparing the CBV value of the same group at different times on the third day and the seventh day,the electroacupuncture group compared with the seventh day on the third day,the CBF value on the seventh day was higher than the third day,the difference was statistically significant(P=0.008< 0.05);There was no statistically significant difference between the third day and the seventh day in the sham operation and model groups(P=0.474>0.05,P=0.842>0.05).MTT: On the third day,the MTT value of the electroacupuncture group was lower than that of the model group and non-acupoint group,and the difference was statistically significant(P=0.000<0.05,P=0.000<0.05);the MTT value of the model group was lower than that of the non-acupoint group.It has statistical significance(P=0.004<0.05).On the seventh day,the MTT value of the electroacupuncture group was lower than that of the model group and non-acupoint group,which was statistically significant(P=0.000<0.05,P=0.011<0.05).On the third day and the seventh day,the MTT value was compared in the same group at different times.Compared with the seventh day on the third day of the electroacupuncture group,the MTT value on the seventh day was higher than the third day,and the difference was statistically significant(P=0.000< 0.05);There was no statistically significant difference between the third day and the seventh day of the sham operation,model group and non-acupoint group(P=0.534>0.05,P=0.861>0.05,P=0.209>0.05).Conclusion: Electroacupuncture at DU26 and ST36 can improve the performance of neurological deficits in MCAO rats,reduce cerebral edema in MCAO rats,reduce the percentage of cerebral infarct volume in MCAO rat models,and increase cerebral blood flow and cerebral blood in the area around MCAO rat infarct lesions The volume and the average passage time of local blood flow are conducive to reducing brain damage after cerebral ischemia-reperfusion,improving the cerebral hypoperfusion state of MCAO rats,and promoting brain tissue repair. |
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