Correlation Between CCR4 And Treg Cells With The Pathogenesis Of Allergic Rhinitis

Objective:Allergic rhinitis?AR?is a widespread nasal allergic disease with an increasing incidence every year.It is one of the common diseases in the otolaryngology department.Quality has a serious impact on people's study,work and life.At present,the preferred treatment strategy for AR is drug therapy.However,according to clinical experience and research,although drug therapy is quite effective in controlling short-term symptoms in most patients,the long-term effects are difficult to satisfy and may cause a variety of adverse effects.reaction.Because its pathogenesis is not completely clear,it is impossible to achieve true causal treatment.In recent years,the research on the pathogenesis of AR has been deepened.The current research focuses on the Th1/Th2 imbalance and Treg/Th17 imbalance and other cell and cytokine levels.Studies have shown that CCR4 and Treg cells are important for the pathogenesis of AR Impact,but the specific mechanism of action is not yet clear.Therefore,this study intends to explore the pathogenesis of allergic rhinitis by detecting the changes of chemokine receptor CCR4,regulatory T cells,CD3⁺T cells,CD4⁺T cells,CD8⁺T cells in the peripheral blood of patients with allergic rhinitis.Methods:From February 2019 to October 2019,30 patients with allergic perennial allergic rhinitis to dust mite were selected as the experimental group,and 30 volunteers were randomly selected as the control group to collect peripheral blood samples.After treatment,the blood samples were labeled with APC-Cy7,FITC,BV510,BV421,BV711 and PE-Cy7 respectively.The CD3⁺T cells and CD4⁺T cells in peripheral blood were analyzed by flow cytometry,CD8⁺T cells and the distribution of CD4⁺CD25⁺ CD127^low/-regulatory T cells and CCR4⁺CD4⁺T cells was detected.After obtaining the two-dimensional scatter diagram,Flow Jo software was used to analyze it.First,two-dimensional scatter diagram was made according to the forward angle scattering light and the lateral angle scattering light as the abscissa and ordinate,and the door was set with lymphocyte group,then two-dimensional scatter diagram was made with APC-Cy7-CD3 and forward angle scattering light as the abscissa and ordinate Fig.CD3⁺T lymphocytes were delineated from the lymphocyte population.Then we use FITC-CD4 and APC-Cy7-CD3 as abscissa and ordinate to make two-dimensional scatter diagram of the detected cells,and calculate the CD4⁺T cells in CD3⁺,the proportion of CD4⁺CD25⁺CD127^low/-in CD4⁺T cells was calculated by using BV421-CD25 and BV711-CD127 as horizontal and vertical coordinates respectively,and then the proportion of CD4⁺CD25⁺CD127^low/-in CD4⁺T cells was calculated by using PE-Cy7-CCR4 and FITC-CD4 as horizontal and vertical coordinates to make two-dimensional scatter diagram of detection cells and CCR4⁺T cells in CD4⁺;Finally,BV510-CD8 and APC-Cy7-CD3 were used as horizontal and vertical coordinates to make a two-dimensional scatter diagram of the detected cells and calculate the proportion of CD8⁺T cells in CD3⁺T cells.The data were analyzed by SPSS 20 statistical software.The percentage of T cells in the experimental group and the control group was expressed as mean±standard deviation??±s?.Independent sample t test was used between the experimental group and the control group?P<0.05?.Results:Compared with healthy volunteers?control group?,the proportion of CD3⁺T cells in peripheral blood of AR group?test group?decreased slightly,but there was no significant difference?P=0.392?;the proportion of CD4⁺T cells in CD3⁺T cells increased,and the difference was statistically significant?P=0.008?;The proportion of CD8⁺T cells in CD3⁺T cells decreased slightly,but the difference was not statistically significant?P=0.104?;the ratio of CD4⁺T/CD8⁺T increased,and the difference was statistically significant?P=0.002?;the ratio of CD4⁺CD25⁺CD127^low/-Treg cells to CD4⁺T cells decreased,and the difference was statistically significant?P=0.004?;the ratio of CCR4⁺CD4⁺T cells to lymphocytes increased,and the difference was statistically significant?P=0.000?.Conclusion:1?During the occurrence of AR,there was no significant change in the overall level of T lymphocytes,but the type and number of T cell subpopulations have changed greatly.The increase of CD4⁺T cells and the imbalance of CD4⁺T/CD8⁺T ratio may have important effects.2?The total number of CD8⁺T cells is not significantly related to the pathogenesis of allergic rhinitis,which may be caused by changes in cell function or imbalance in the distribution of internal subgroups;3?The occurrence and development of allergic rhinitis are significantly related to the reduction of CD4⁺CD25⁺CD127^low/-Treg cells,which may be related to the weakened immune suppression function;4?CCR4 is closely related to the imbalance in the distribution of Th1/Th2 cell subsets,which is significantly related to the pathogenesis of allergic rhinitis,or it can be a new target for clinical treatment of allergic rhinitis.