Effects Of Probiotics On Giucose And Lipid Metabolism And Insulin Resistance In In Type 2 Diabetic Rats

Objective: The purpose of this study was to investigate the effects of probiotics(Bifidobacterium quadruple live bacteria tablets)on glucose and lipid metabolism and insulin resistance in type 2 diabetic rats,and to explore whether its potential mechanism of action is related to improving inflammation in the body.Methods: Forty SD male rats were randomly divided into normal control group(N group n = 8)and diabetic model(n = 32)according to body weight,and were fed with ordinary feed and high-fat diet,respectively.After 4 weeks,the diabetic model rats induced a type 2diabetes model with low-dose streptozotocin(STZ).Seventeen type 2diabetic rats successfully modeled were randomly divided into a diabetes model group(M group,n = 8)and a probiotic intervention group(T group,n = 9).Group T was given probiotics(2g / d),and other groups were given saline.During the experiment,body weight(BW),fasting blood glucose(FBG),and postprandial blood glucose(PBG)weremeasured once a week.One day before the end of the experiment,rats in each group were administered an oral glucose tolerance test(OGTT).After the end of the 4-week experimental cycle,blood was taken from the abdominal aorta to measure the fasting blood glucose(FBG)and fasting insulin(INS)levels of each group and calculate the insulin resistance index(HOMA-IR),and measure the triglycerides(TG)and total cholesterol(TC),Low-density lipoprotein(LDL-C),high-density lipoprotein(HDL-C),tumor necrosis factor-?(TNF-?),and interleukin-6(IL-6)levels.Results: 1.Compared with N,FBG,PBG,HOMA-IR,TG,LDL-C of group M diabetic rats were significantly increased(P <0.05),BW and HDL-C were significantly reduced(P <0.05),TC No significant difference(P>0.05).Compared with the M group,the levels of FBG,PBG,HOMA-IR,TG,TC,LDL-C in the T group were significantly decreased(P <0.05),BW was increased(P <0.05),and there was no significant difference in HDL-C levels(P <0.05).2.Compared with N,the levels of TNF-? and IL-6 in M group rats increased significantly(P<0.05).Compared with the M group,the levels of TNF-? and IL-6 in the T group increased(P <0.05).Conclusion: Probiotics can ameliorate glucose and lipid metabolism disorders and insulin resistance in type 2 diabetic rats.The underlyingmechanism may be related to the reduction of inflammatory reactions in vivo by probiotics.