Expression And Clinical Significance Of Chemokine CCL2 In Oral Squamous Cell Carcinoma

Objective: To study the relationship between the expression and change of chemokine CCL2 and the recurrence and metastasis of oral squamous cell carcinoma.Method: Some eligible tissue samples of patients with oral squamous cell carcinoma who underwent surgery in the department of head and neck of Hunan Cancer Hospital from June 2018 to January 2019 were collected.Including 58 patients' paraffin cancer tissue and blood samples(before and after the surgery),and 14 patients' fresh cancer and paracancerous tissue.Immunohistochemical method was used to detect the expression of CCL2 protein,the expression difference of CCL2 in oral squamous cell carcinoma and adjacent tissues was analyzed,and the relationship between CCL2 and clinical pathology of oral squamous cell carcinoma was analyzed.ELISA was used to detect the CCL2 content in peripheral blood of patients before and after the operation.The relationship between CCL2 and recurrence and metastasis of oral squamous cell carcinoma was analyzed.SPSS 23.0 was used to analyze the data.Result: 1.There was a significant difference(p < 0.0001)between the positive expression of CCL2 in oral squamous cell carcinoma tissues(78.6%)and in adjacent tissues(21.4%).2.The relationship between the positive expression of CCL2 and T stage in oral squamous cell carcinoma is shown in 47 cases of T1-2 stage,with 46.8% positive expression and 53.2% negative expression.In 25 cases of T3-4,positive expression was 88%,negative expression was 12%(p < 0.05).The relationship between the positive expression of CCL2 and he degree of differentiation showed in the highly differentiated group(54 cases),with 53.7% positive expression and 46.3% negative expression,and in the medium-low differentiation group(18 cases),with positive expression accounting for 83.3% and negative expression accounting for 16.6%(p < 0.05).The relationship between the positive expression of CCL2 and lymph node metastasis was shown in 39 cases of N-group,with positive expression accounting for 43.6% and negative expression accounting for 56.4%.There were 33 cases in N+ group,with 81.8% positive expression and 18.2% negative expression(p < 0.05).The relationship between the positive expression of CCL2 and clinical stages showed in stage I-II group(26 cases)had positive expression of 26.9% and negative expression of 73.1%,and in clinical stage III-IV group(46 cases),the number of positive expression and negative expression were 80.4% and 19.6%;(p < 0.05).There was no significant correlation with sex,age and tumor recurrence(p > 0.05).3.The post-and pre-operative peripheral blood CCL2 content were compared,the postoperative peripheral blood CCL2 content was significantly lower than that before operation(p < 0.05).In further study among T1-2 group,highly differentiated group,N+ group,I-II group and non-recurrent patients,the postoperative peripheral blood CCL2 content of patients in recurrence group was significantly higher than that in the pre-operation stage(p < 0.05),while the postoperative peripheral blood CCL2 content of patients in T3-4 group,medium and low differentiation group,N-group and III-IV group was not significantly higher than that in the pre-operation stage(p > 0.05).Conclusion: 1.the expression of CCL2 protein in oral squamous cell carcinoma is higher than that in adjacent tissues.CCL2 expression is related to tumor invasion,differentiation degree,clinical stage and lymph node metastasis.2.After surgical resection of solid tumors,the concentration of CCL2 in peripheral blood shows a decreasing trend compared with that before surgery.If CCL2 expression in postoperative peripheral blood is still higher than that before operation,it indicates that the possibility of tumor recurrence is high.