Cytoglobin Inhibits Cardiomyocyte Apoptosis By Upregulating The Expression Of Mipu1

Objective:The role cytoglobin on cardiomyocyte apoptosis induced by oxidative stress and its possible mechanism.Methods:H9c2 cells were treated with 500?M H₂O₂ for different time?6 h,12 h and 24 h?.Then,H9c2 cells were treated with different concentrations of H₂O₂?250?M,500?M and 1000?M?for 12 h.The expression of CYGB in H9c2 was detected by western blot.The transfection efficiency of CYGB-shRNA lentivirus into H9c2 was analyzed by western blot and fluorescence microscope.The effect of CYGB-shRNA on the LDH activity of H9c2 cells induced by H₂O₂ was detected by Elisa.The effect of CYGB-shRNA on the survival rate of H9c2 cells induced by H₂O₂ was detected by MTT.Flow cytometry was used to detect the effect of CYGB-shRNA on the apoptosis rate of H9c2cells induced by H₂O₂,and the effect of CYGB-shRNA on the expression of Casepase-3 in H9c2 induced by H₂O₂ was detected by western blot.MTT,flow cytometry and western blot were used to analyze the effect of inhibition of CYGB expression and upregulation of Mipu1 expression on the survival rate and apoptosis rate of H9c2 cells.Results:H9c2 treatment with different time and concentration could upregulate the expression of CYGB.The expression of CYGB in H9c2treated with 500?M H₂O₂ for 12 hours was the most obvious.Transfection of CYGB-shRNA could significantly inhibit the expression of CYGB in H9c2.Inhibition of CYGB expression decreased the survival rate of H9c2 cells induced by H₂O₂,but increased the apoptosis rate,LDH activity and Casepase-3 expression,the above effects can be partly antagonized by upregulating the expression of Mipu1 at the same time.Conclusion:CYGB can antagonize the apoptosis of H9c2 cells induced by H₂O₂,which related to the upregulation of Mipu1 expression.