UVRAG Is Involved In The Process Of Ferroptpsis In K562 Leukemia Cells

Objective:To explore whether UV radiation resistance-associated gene involved in the process of ferroptosis in K562 leukemia cells.Methods:1.According to the different concentrations of Erastin(10 m mol/L,20mmol/L,40mmol/L,60mmol/L,80mmol/L),the K562 leukemic cells were divided into experimental groups and blank control group(equivalent PBS solution).The intracellular Fe2+ concentration of K562 leukemic cells was detected at 24 hour,48 hour and 72 hour respectively to evaluate the process of ferroptosis.Combined with cell morphology,the best administration concentration and treatment time were found.2.K562 leukemic cells were divided into three groups:(Erastin)group,inhibition group(Liproxstatin-1)and blank control group.The expression of UVRAG protein in different groups was detected by Western Blot.3.K562 leukemia cells si RNA-UVRAG group,si RNA-UVRAG+Erastin group,induction group(Erastin)and blank control group were set up,and iron kits were used to evaluate ferroptosis in different groups.Results:1.When K562 leukemic cells were treated with Erastin,with the increase of Erastin concentration,the number of K562 cells decreased significantly,the cell morphology was irregular,the cell membrane was deformed,the cell debris increased,the cell activity decreased,and the serum iron concentration gradually increased,indicating that the degree of iron death was increased,which was the most obvious in 48 hours and40mmol/L Erastin group(P<0.05).2.Compared with the blank group,the expression of UVRAG protein in the induction group was up-regulated(P<0.05),and the expression of UVRAG protein in the inhibition group was downregulated compared with the blank group(P<0.05).3.There was no significant difference in the serum iron concentration between the si RNA-UVRAG group and the blank control group(P>0.05),and there was no significant difference in the serum iron expression between the si RNA-UVRAG group and the si RNA-UVRAG+Erastin group(P<0.05).Compared with the induction group,he serum iron in the si RNA-UVRAG+Erastin group was down-regulated(P<0.05).Conclusion:1.The appropriate concentration of Erastin to treat K562 leukemic cells was 40mmol/L,and the optimal time was 48 hours.2.UVRAG participates in the process of ferroptosis in K562 leukemia cells.