The Expression Of WISP1 In Esophageal Cancer Cells Its And Effect On The Proliferation And Migration Of Cancer Cells

Background The incidence rate of esophageal cancer is increasing.Lymph node metastasis is an important way of esophageal cancer metastasis.Regional lymph node metastasis is the main cause of tumor recurrence and distant metastasis,and also an important factor of poor prognosis.Wnt1 inducing pathway protein 1(WISP1)is a secreted extracellular matrix related protein.It has been found that WISP1 expression is abnormal in some tumors,and it is closely related to the clinicopathology and survival prognosis of tumor patients,which is expected to become the target of tumor diagnosis,treatment and prognosis.However,the expression and function of WISP1 in esophageal cancer cells is remain unclear.In the present study,the expression level of WISP1 gene in esophageal cancer cells and its impact on the proliferation of cancer cells were investigated.The findings in this paper may provide a new strategy for the treatment and prognosis of esophageal cancer.Objective(1)To study the expression of WISP1 gene in human esophageal cancer cells;(2)Effect of RNAi inhibition of WISP1 gene expression on the proliferation of esophageal cancer cells;(3)Effect of RNAi inhibition of WISP1 gene expression on colony formation of esophageal cancer cells;(4)Effect of RNAi inhibition of WISP1 gene expression on cell migration of esophageal cancer cells.Methods(1)The normal esophageal epithelial HET-1A cell line and esophageal cancer EC9706 cell line were cultured,and the real-time fluorescence quantitative PCR(q PCR)was used to detect the relative expression of WISP1 m RNA; (2)the specific sh RNA target of the WISP1 gene was designed;Esophageal cancer cells were stained to construct suppressive expression cells of WISP1 gene.The m RNA level of WISP1 in transfected cells was detected by q PCR method to identify the gene suppression effect.;(3)MTT method was used to detect cell proliferation ability;clone formation test was used to detect cell clone formation ability;cell scratch test was used to detect cell migration ability.Results(1)The q PCR results showed that the relative expression of WISP1 m RNA in esophageal cancer cell EC9706 was significantly higher than that of normal esophageal epithelial cells(P <0.05).(2)The results of q PCR showed that the expression of WISP1 m RNA in sh RNA treated with wisp78 and wisp79 was significantly lower than that in NC group.(3)After sh RNA lentiviral vector transfection,the inhibition rate of WISP78 and WISP79 cells reached 30%,and the effect was significant;indicating that the sh RNA can affect the cell proliferation.(4)After the same amount of 500 cells were spread into the pore plate,crystal violet staining was carried out on the tenth day of the experiment.The figure above shows that the clonal formation of cells in the experimental group was significantly slower than that in the control group.(5)The results of scratch test showed that the migration rate of cells in the experimental group was significantly slower than that in the control group at the beginning of 12 hours,until the cells in the control group almost completely fused at 48 hours,and the experimental group showed significant inhibition.Conclusion WISP1 protein is highly expressed in esophageal cancer cells.RNAi can inhibit the expression of WISP1 gene m RNA in esophageal cancer cells.Inhibition of WISP1 gene can inhibit the proliferation,clone formation and cell migration ability of esophageal cancer cells.