| Objective: To observe the intervention of Compound Glycyrrhizin on nonalcoholic fatty liver disease model in rats and explore its possible mechanismMethods: twenty six SD rats were selected,and randomly divided into high fat diet group and normal control group.There were 16 rats in the high fat diet group fed with high fat diet,and 10 rats in the normal control group fed with normal diet.After 16 weeks,we randomly killed 4 rats in each group.Hematoxylin-eosin(HE)staining was used to deal with the hepatic tissues,which helped to evaluate the modeling progress.After confirmed NAFLD rat modle,the remaining 18 model rats in high fat diet group were randomly divided into 2 equal groups,model group and compound glycyrrhizin group.Compound glycyrrhizin group was given Compound glycyrrhizin by intragastric gavage,normal group and model group were given the same amount of physiological saline for 4 weeks.The serum of aspartate aminotransferase(AST),alanine aminotransferase(ALT),triglycerides(TG),and total cholesterol(TC)were detected by automatic biochemical analyzer.,Enzyme linked immunosorbent assay(Elisa)was used to measure the superoxide dismutase(SOD),malondialdehyde(MDA),interleukin-1β(IL-1β),interleukin-6(IL-6),and tumor necrosis factor-α(TNF-α).SOD,MDA,and TNF-α in liver tissues were also measured.With the help of HE staining,we can observe the histological changes of liver tissues.After treated with compound glycyrrhizin.Western blot was used to determine the expression of AMPK,SIRT1,and UCP2 protein in liver tissue of rats.Real-time quantitative RT-PCR was used to determine the expression of AMPK,SIRT1,and UCP2 m RNA in liver tissue of rats.Results:1.Hepatic pathological changes: In the model group,The hepatic lobular structure was disordered,and a large number of lipid vacuoles were seen in the hepatocyte.the hepatic cord radially arranged structure can be seen after treated with compound glycyrrhizin,compared with the model group,fatty vacuoles were significantly reduced,indicating that compound glycyrrhizin improved liver steatosis in NAFLD rats;2、Changes in biochemical indicators:Compared with the normal group The serum levels of TG,TC,ALT、AST in the model group were significantly increased(P <0.05);Compared with the model group,the levels of serum ALT and AST in the compound glycyrrhizin group were decreased(P <0.05),the levels of serum TG and TC were decreased(P >0.05),suggested that compound glycyrrhizin can improve liver function damage of NAFLD to a certain extent.3、Changes of inflammatory factors and oxidative stress indicators: Serum SOD levels and hepatic SOD levels decreased significantly in model group than that in normal group,serum MDA and TNF-α levels and hepatic MDA and TNF-α levels increased,and serum IL-1β and IL-6 levels also increased in model group.Compared with model group,serum SOD levels and hepatic SOD levels increased significantly in compound glycyrrhizinl group,serum MDA and TNF-α levels and hepatic MDA and TNF-α levels decreased,and serum IL-1β and IL-6 levels also decreased in compound glycyrrhizinl group.,the difference was statistically significant(P<0.05).This demonstrates that compound glycyrrhizin may inhibit oxidative stress and inflammatory response in non-alcoholic fatty liver disease.4、Gene and protein changes of AMPK,SIRT1,UCP2:Compared with normal group,The expression of AMPK and SIRT1 gene and protein in model group were significantly decreased,while the UCP2 gene and protein expression were significant increased(P < 0.05).Compared with model group,The expression of AMPK and SIRT1 gene and protein were significantly increased in compound glycyrrhizin group,while the UCP2 gene and protein expression were significantly reduced(P<0.05).The results suggest that the compound glycyrrhizin could treat NAFLD by activating the expression of AMPK and SIRT1 and inhibiting the expression of UCP2.Conclusion:1、Compound glycyrrhizin can improve hepatic steatosis in NAFLD rats,which has a therapeutic effect.2、Compound glycyrrhizin may have a effect on oxidative stress and inflammatory response of non-alcoholic fatty liver disease.3 、 Compound glycyrrhizin may participate in the process of lipid metabolism and oxidative stress in non-alcoholic fatty liver disease and play an anti-NAFLD effect by regulating the AMPK / SIRT1 / UCP2 pathway... |
