| Objective: To investigate the expression and significance of SNHG3 gene in adenomyosis.Methods: 1.Collection of clinical specimens All samples were obtained from Yanjishan Hospital of Wannan Medical College.The patients who participated in this study have signed the informed consent with the approval of the Ethics Committee of Jijishan Hospital.Endometrial tissues from 42 patients with adenomyosis and 50 normal controls were collected.The pathological results were verified by the Department of Pathology.Specimens were stored in-80 ? liquid nitrogen before RNA extraction.No hormonal treatment was performed before all specimens were collected.2.RNA extraction,reverse transcription and real-time PCR TRIzol method was used to extract total RNA from tissues,reverse transcribe it into total cDNA,and perform qRT-PCR experiments to detect mRNA expression levels of SNHG3 gene in ectopic endometrium of adenomyosis group and normal endometrium of control group.3.Western Blot Technology Western Blot technique was used to detect ectopic endometrium of adenomyosis group and normal endometrium of control group,respectively.Protein expression level of SNHG3 gene.Results: 1.Expression of SNHG3 in adenomyosis1.1 Detection of target gene expression by qRT-PCR:(1)The expression of SNHG3 was 10.37±1.58 in the adenomyosis group and 8.66±2.72 in the normal control group.Compared with the two groups,the expression of SNHG3 gene was higher in the adenomyosis group than in the control group.There was statistical significance(t = 3.19,P = 0.002,P <0.05).(2)2238bp mRNA expression of SNHG3 transcript in the adenomyosis group was 14.67±0.32,and 2238 bp mRNA expression of SNHG3 transcript in normal control group was 12.79±0.30.Compared with the SNHG3 gene transcript,2238 bp in adenomyosis MRNA expression in ectopic endometrial tissue was higher than that in the control group,and the expression difference was statistically significant(t = 4.33,P = 0.001,P <0.05).(3)In the adenomyosis group,the expression of 2346 bp mRNA of SNHG3 transcript was 9.89±0.26,and the expression of 2346 bp mRNA of SNHG3 transcript in control group was 9.43±0.40.There was no significant difference in expression(t = 0.92,P = 0.362,P> 0.05).1.2 Western Blot technology to detect protein expression:The expression of SNHG3 gene protein RCC1(6.27±0.52)in the adenomyosis group was significantly upregulated compared with the control group(4.62±0.40),and the difference was statistically significant(t = 3.085,P = 0.018,P <0.05).2.General information comparison: A total of 92 clinical data were collected in this experiment.There were 42 patients in the adenomyosis group.The patients were 27 to 50 years old,with an average age(34±2.7)years.All patients had no other medical history,and had not used hormone drugs for 6 months before surgery.There were 50 cases in the normal control group,ranging in age from 20 to 52 years,with an average age(35.8±3.7)years.All normal controls had no endometrium treatment for 6 months and had no other disease history.Conclusion: 1.The expression of SNHG3 transcript 2238 bp was higher in the adenomyosis group than in the control group,suggesting an effect on the occurrence of adenomyosis.2.SNHG3 mRNA expression in ectopic endometrium of adenomyosis group was higher than that of control group,suggesting that SNHG3 is involved in the development of adenomyosis.3.The protein expression of SNHG3 gene in ectopic endometrium of adenomyosis is higher than that of the normal endometrium of the control group,suggesting that the SNHG3 gene is related to the pathogenesis of adenomyosis.In summary,the research in this experiment in adenomyosis shows that SNHG3 may be a new therapeutic target.Further research on the SNHG3 gene,such as bioinformatics analysis,functional research,and other studies combined with clinical analysis may become a new clinical treatment direction. |
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