Correlation Between HDL,APOA1 And Inflammatory Bowel Disease Activity And Its Effect On Experimental Enteritis In Mice

Inflammatory bowel disease(IBD)is a chronic inflammatory disease with complex etiology that can affect the entire digestive tract.It can be divided into two main types:Crohn's disease(CD)and ulcerative colitis(UC).Studies have confirmed that the inflammatory can aggravate the imbalance of lipid metabolism,and at the same time lipid metabolism can affect the inflammation.This study mainly explored the correlation between serum high-density lipoprotein(HDL)and its main functional protein Apolipoprotein A1(APOA1)levels and clinical inflammatory activity of IBD,as well as the effects of HDL,APOA1 on acute mice enteritis induced by dextran sulfate sodium salt(DSS).This article is divided into two parts:Chapter I:Correlation between serum HDL and APOA1 levels and inflammatory activity of inflammatory bowel diseaseObjective:This section studies the changes of serum HDL and APOA1 levels in CD and UC patients with inflammatory bowel disease and their correlation with the degree of inflammatory activity.Methods:A retrospective study was conducted on the serum HDL and APOA1 levels of inpatients with inflammatory bowel disease hospitalized in the Affiliated Hospital of Yangzhou Universty from January 2013 to August 2019.Take the person without physical examination as the control group,compare the difference in blood lipid levels between the two groups and observe the relationship between HDL and APOA1 levels and some inflammation indicators such as C-reactive protein(CRP),Erythrocyte sedimentation rate(ESR).Results:A total of 228 patients with IBD were included(147 in CD and 81 in UC).The levels of serum HDL and APOA1 in patients with IBD were significangly lower than those in healthy controls(both P values<0.01).Serum HDL and APOA1 levels were negatively correlated with C-reactive protein(CRP)and Erythrocyte sedimentation rate(ESR)levels in patients with IBD(both P values<0.001).Conclusion:The levels of serum HDL and APOA1 in patients with IBD were significantly reduced,which was inversely related to the degree of inflammatory activity.Chapter ?:Effects of HDL and APOA1 on DSS-induced acute enteritis in miceObjective:This section investigates the effect of HDL and its main functional protein APOA1 on DSS-induced acute enteritis in mice.Methods:First use 3%DSS to induce acute enteritis model in mice.HDL concentrations of lOug,100ug,and 500ug were injected intraperitoneally to observe the protective effects of the drugs,and a control group was set up to observe the changes in body weight,stool consistency,and blood in the stool in each group.The colon tissue of mice was stained with hematoxylin and eosin,and the pathological changes were observed under a microscope.Secondly,APOA1 KO and wild type(WT)control mice were used to establish a mouse model of acute enteritis,and the changes in body weight,stool consistency,and blood in the stool were observed in each group.After seven days,the intestinal tissue of mice was taken for flow cytometry to detect the effect of APOA1 on the expression of CD4,CD8 and CD44 cells.Results:HDL can prevent DSS-induced acute colitis in mice.HDL can reduce the inflammation of colon in mice.The delection of APOA1 gene can aggravate the acute enteritis in mice induced by DSS.The deletion of APOA1 gene can promote the migration of T cells to the intestine and promote the pathogenesis of DSS-induced enteritis.Conclusion:HDL and its main functional protein APOA1 can protect DSS-induced acute colitis in mice.