| BackgroundOsteoarthritis is a common bone and joint disease in clinical work.The disease often involves the knees and hips,with joint swelling and pain as its main symptoms.In severe cases,joint deformities can cause movement disorders,which greatly affects the quality of life of patients.With the continuous exploration of the disease,studies have found that low-level inflammatory response and metabolic disorders are important links in the occurrence and development of osteoarthritis.At the same time,through high-throughput sequencing,it was found that the species and composition of gut microbiome were out of balance,which may lead to metabolic disorders and inflammatory reactions through immune regulation and the increase of lipopolysaccharide concentration in circulation..This suggests that there is a potential relationship between the disorder of gut microbiome and osteoarthritis.However,there is still a lack of large sample data to prove the relationship between osteoarthritis and gut microbiome.ObjectiveThe purpose of this study is to analyze the differences in the composition of gut microbiome and bacterial species between patients with osteoarthritis and normal people,hoping to find the unique composition and representative bacteria of patients with osteoarthritis.In addition,the functional prediction was used to analyze the mechanism of the changes of gut microbiome in the occurrence and development of osteoarthritis.Methods(1)A case-control study was conducted in the first affiliated Hospital of Zhengzhou University from March 2018 to March 2019.According to the symptoms,signs and imaging data,56 patients with osteoarthritis(34 females)and 53 healthy subjects were selected as the control group(52 patients were actually included in the control group,including 28 females).Stool samples were collected from the above individuals.(2)High-throughput sequencing of 16S rRNA gene was used to analyze the classification and composition of gut microbiome in patients with osteoarthritis,and then LEfSe analysis was used to find out the main differential bacteria and biomarkers in patients with osteoarthritis.(3)PICRUSt analysis was used to predict the genomic function of gut microbiome in patients with osteoarthritis,then we explored its mechanism in the occurrence and development of the disease.Results(1)There was no significant difference in the richness and evenness of gut microbiome between the osteoarthritis group and the healthy control group(P>0.05).However,there was a significant difference in the composition of gut microbiome between the osteoarthritis group and the healthy control group(P<0.05).(2)There were significant differences in the composition of gut microbiome between the osteoarthritis group and the healthy control group at different levels(phylum,class,order,family,genus,species).Actinobacteria,Bifidobacteriales,Bifidobacteriaceae and Bifidobacterium were significantly enriched in osteoarthritis patients.However,the abundance of Prevotellaceae was significantly lower than that of the normal population(P<0.05).There was a significant correlation between the abundance of appeal differential bacteria and BMI index(P<0.05).(3)PICRUSt analysis showed that gut microbiome in patients with osteoarthritis was related to glucose metabolism,fat metabolism,fat metabolism,pro-inflammatory response and intercellular signal transmission.Conclusion(1)There were significant differences in the composition of gut microbiome between patients with osteoarthritis and normal people at all levels(phylum,class,order,family,genus and species).(2)The abundances of Actinomycetes,Actinomycetes,Bifidobacteria,Bifidobacteriaceae and Bifidobacterium were significantly different from those of healthy controls.Bifidobacterium,Lactobacillus and Prevotellaceae could be used as a biomarker for osteoarthritis..(3)Bifidobacterium,Lactobacillus and Prevotellaceae were related to the pathogenesis of osteoarthritis and could be used as targets for disease prevention and treatment. |
PDF Full Text Request