| Objective: To investigate the effect and mechanism of continuous intravenous infusion of lidocaine or ulinastatin on inflammatory response and acute lung injury in sepsis rats.Methods: 60 male SD rats were randomly divided into four groups(n=15): sham operation group(S group),sepsis group(Sep group),ulinastatin group(U group)and Lidocaine group(L group).rats were sacrificed to collect blood from the inferior vena cava 24 hours after CLP.Liver function(ALT,AST),renal function(Cr),lactic acid level,IL-6,TNF-?,HMGB1 and HMGB1 mRNA expression in lung tissue were measured in each group.The pathological changes of lung tissue were observed by light microscope,and the lung wet/dry weight ratio(W/D)was calculated.In addition,40 rats were selected to observe the 72-hour survival after CLP according to the above groups(10 in each group).Results: Compared with the S group,the ratios of liver function(ALT,AST)and renal function(Cr),lactic acid level,serum IL-6,TNF-?,HMGB1,and HMGB1 mRNA expression in lung tissue,in Sep group and U group L group were increased(P<0.05).Compared with Sep group,all indexes of U group and L group were decreased(P<0.05).The pathological changes in the U and L groups were significantly reduced compared with the Sep group.The 72-hour survival rate of U and L groups after CLP was significantly higher than that in the Sep group.There is no significant difference in the indicators between groups U and L.(P> 0.05).Conclusion: Sepsis can enhance the expression of proinflammatory factors in rats.Continuous intravenous pumping of lidocaine and ulinastatin can effectively reduce the expression of various inflammatory factors in rats with sepsis,and at the same time inhibit the HMGB1 mRNA gene in lung tissues.Continuous intravenous pumping of lidocaine and ulinastatin can reduce the damage of sepsis to the lungs and effectively improve the survival rate of animals.Lidocaine's efficacy in reducing sepsis inflammation and lung protection is similar to that of ulinastatin. |
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