| Objective:To investigate the clinical significance of the expression of integrin β2protein and tumor infiltrating dendritic cells CD80,CD86 and ICAM-1 in TNBC(Triple negative breast cancer).Methods:The expression levels of ITGB2 and MHC-1 molecules in MDA-MB-231 cells and TNBC tissues were detected by Western bolt and RT-PCR,and the expressions of ITGB2,CD80,CD86 and ICAM-1 in TNBC tissues were detected by immunohistochemistry.Results:1.Compared with normal breast cell line,the expression level of ITGB2 in TNBC cell line was significantly higher(P<0.05),and the expression level of MHC-1 molecules in TNBC cell was significantly lower than that in normal breast cell line(P<0.05).2.The expression level of ITGB2 in TNBC breast tissue was significantly higher than that in normal breast tissue(P<0.05).3.Immunohistochemical staining showed that the expression level of ITGB2 in 148 cases of TNBC was significantly higher than that in paracancerous tissues(P<0.05).The clinicopathological data showed that the expression of ITGB2 in TNBC was correlated with tumor stage,histological grade,lymph node metastasis,ki67 expression and total survival(P<0.05).4.Immunohistochemical staining showed that the expression of CD80 and CD86 in tumor infiltrating dendritic cells in TNBC was lower than that in paracancerous tissues,and the expression of ITGB2 ligand ICAM-1 was significantly higher than that in paracancerous tissues(P<0.05).Conclusion:High expression of ITGB2 and ICAM-1 and low expression of MHC-1 molecules,CD80 and CD86 were found in TNBC.The high expression of ITGB2 affects the prognosis of patients with TNBC. |
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