The Studies On The Expression Of LncRNA-n334788 In Idiopathic Pulmonary Fibrosis

Idiopathic pulmonary fibrosis(IPF)is a chronic and progressive fibrotic interstitial pneumonia of unknown etiology and poor prognosis.Currently,there is still no effective treatment for IPF.Emerging studies have confirmed that lncRNAs are involved in regulating the pathophysiological processes of various human diseases,and lncRNAs have become one of research hotspots in recent years.However,the role of lncRNAs in IPF has not fully elucidated.Objectives:To explore the expression level of lncRNA-n334788 in IPF.Methods:The GeneChipTM Human Transcriptome Assay 2.0 was used to select lncRNAs that were significantly different expressed in the peripheral plasma of IPF patients and healthy controls(HCs),and real-time fluorescence quantitative PCR(qRT-PCR)was used to verify the expression level of lncRNAs.FISH was used to detect the expression level of lncRNA-n334788 in the lung tissues from IPF patients and subcelluar localization of lncRNA.In vitro,type Ⅱ human alveolar epithelial cells A549 were used to construct an epithelial mesenchymal transition(EMT)cell model mimic IPF.The expression level of lncRNA-n334788 in A549 cells was detected by qRT-PCR.Results:According to the data of gene chip,the expression of lncRNA-n334788 in the peripheral plasma of IPF patients was markedly higher than that in the peripheral plasma of HCs.FISH revealed that lncRNA-n334788 was distributed in the cytoplasm,and its expression in lung tissues from IPF patients was significantly increased compared with that from controls.Subsequently,in vitro,we found that the expression of lncRNA-n334788 was also obviously upregulated in the TGF-β1-induced A549 cell EMT model.Conclusion:This study firstly revealed the expression level of lncRNA-n334788 in idiopathic pulmonary fibrosis.The results showed that lncRNA-n334788 was highly expressed in IPF patients and in A549 cell EMT model.