| ObjectiveBy randomized controlled clinical trials explore drinking hydrogen-rich water as well as the implementation of health education intervention on carotid atherosclerosis patient clinical effect,in order to find new treatments for the disease.MethodAccording to the diagnostic criteria of Chinese experts recommendation for diagnosis and treatment of carotid atherosclerotic disease in 2013,a total of 46 eligible CAS patients were selected from the Department of Cardiology of a 3A hospital in Tai'an.The selected patients were randomly divided into 2groups,the experimental group and control group with 23 patients each.The experimental group was treated with hydrogen-rich water and routine health education interventions.The control group received only routine health education interventions.All patients signed informed consent according to the requirements.Each patient of the experimental group drinks hydrogen-rich water at a dose of 900mL/day(drink 300mL at morning,noon and evening,respectively).After being treated for 0 weeks and 12 weeks,fasting venous blood was collected to carry out the following test.1.Health-promoting Lifestyle Profile?(HPLP?)and Morisky drug compliance ques-tionnaire(MMAS-8)were investigated.2.Plasma biochemical examination:fasting venous blood collected from CAS patients was sent to Affiliated Hospital of Tai'an Medical College for testing total cholesterol(TC),low density lipoprotein cholesterol(LDL-C),triglyceride(TG)and high-density lipoprotein cholesterol(HDL-C).3.Color Doppler ultrasound examination of carotid artery intima-media thickness(IMT)of all the patients were performed using Germany SIEMENS S2000 Doppler ultrasound instrument examination with linear array probe,7~10 MHz frequency.The patient was asked to take supine examination,exposure measurement site,and head to the opposite direction of the detection site slightly.The IMT value of the carotid artery was measured by bilateral mean value.4.Oxidized low density lipoprotein:before and after the intervention,we used the oxidized low density lipoprotein kit of Shanghai enzyme linked biotechnology limited company to measure the concentration of ox-LDL in plasma of CAS patients.5.Flow data detection of endothelial progenitor cells:the ratio of KDR1+CD34~+an KDR2+CD133~+of endothelial progenitor cells before and after intervention were detected respectively.ResultsThe scores of HPIP and Morisky scale in the health education intervention group were significantly higher than those before the intervention.It means that the health of the patients in the experimental group improves the way of life and the compliance of the medicine.The outcome of the simple health education group was slightly better than that before the intervention,but the results were not as significant as that in the experimental group.The results of blood lipid showed that the TC level,ox-LDL and low density lipoprotein(LDL)level in the experimental group were significantly lower than those in the experimental group,while the levels of high-density lipoprotein(HDL)and triglyceride(TG)did not improve significantly.Flow cytometry showed that the kdr1+CD34~+double positive ratio and kdr2+CD133~+double positive ratio of the experimental group were significantly increased.The results of carotid artery color Doppler ultrasound showed that the thickness of the middle membrane(IMT)of the patients in the experimental group was significantly better than that of the control group.Conclusions1.Hydrogen-rich water can improve the health promoting lifestyle of CAS patients and improve the compliance of patients.2.Hydrogen-rich water can improve blood lipid level,reduce plasma total cholesterol level,low density lipoprotein level and oxidized low density lipoprotein level in CAS patients.3.Hydrogen-rich water can increase the number of peripheral blood endothelial progenitor cells in CAS patients.4.Hydrogen-rich water can improve the carotid intima-media thickness in CAS patients. |
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