| Objective:To investigate the differences in the expression of angiogenic factors and the expression of angiogenesis factor protein and mRNA in hippocampus of depressed rats.To explore the relationship between the degree of depressive disorder and angiogenesis factors,and to provide experimental evidence for the pathogenesis of depression,especially late-onset depression.Methods:The study is divided into two parts:clinical research and basic research:Part one:clinical study:A total of 253 people were enrolled,and were randomly divided into 143 patients in the depression group and 110 patients in the normal control group.The serum levels of vascular endothelial growth factor(VEGF),hypoxia inducible factor-1(HIF-1)and alkaline formation were determined by enzyme-linked immunosorbent assay(ELISA).Basic fibroblast growth factor(bFGF).The results of the two groups of pro-angiogenic factors were divided into four groups:one group:18 years or older and less than 30 years old;2 groups greater than or equal to 30 years old and less than 40 years old;3 groups greater than or equal to 40 years old and less than 50 years old;4 groups greater than or equal to 50 years old is less than or equal to 60 years old for comparisonThe second part:basic study:Forty-eight SPF male SD rats(3M)were randomly divided into fluoxetine group(n=16),depression model group(n=16),and control group(n=16).The control group did not receive any treatment,and was free to eat and water for 5 weeks.The rats in the other groups were subjected to a chronic mild and unpredictable multi-correspondence method for 21 days to establish a depression model.After modeling,the fluoxetine group was given fluoxetine for 2 weeks.At the end of the 5th week of the experiment,all the rats(decapitation of the rats were directly killed,and the hippocampus was taken out on an ice bath)were subjected to Western blotting and reverse transcription-polymerase chain reaction(RT-PCR)experiments.Results:1.Clinical study:1)Compared with the normal control group,the HIF1(1.738±0.880)in the depression group was higher than that in the normal control group(1.169 ± 0.599)(P<0.05),depressive disorder.The VEGF of the group(1.008±0.816)was lower than that of the normal control group(1.737±0.822)(P<0.05),and the difference of bFGF(0.048 ± 0.004)between the depressive disorder group and the normal control group bFGF(0.047 ± 0.003)was not statistically significant.(P>0.05).2)Comparison within the group:1 In the group of patients with depressive disorder,a.Compared with the expression of VEGF(0.704±0.598)in the group of patients older than 40 years old and younger than 50 years old,the VEGF of the patient group greater than or equal to 18 years old and younger than 30 years old(1.093)VEGF(1.083±0.732)expression was increased in patients with greater than or equal to 30 years of age and younger than 40 years old,the difference was statistically significant(P<0.05);b.and VEGF(0.481)in patients with age greater than or equal to 50 years old and less than 60 years old.v 0.362)expression was higher than or equal to VEGF in patients aged 18 years and younger than 30 years old and VEGF expression in patients older than 30 years old and younger than 40 years old,the difference was statistically significant(P<0.05)C.There was no significant difference in HIF-1 and bFGF between patients of all ages(P>0.05).2 In the normal control group,a.Compared with the expression of bFGF(0.048 ± 0.002)in the group of 40 years old and younger than 50 years old,the bFGF(0.047 ± 0.002)and the group of 30 years old or younger than 30 years old and less than 30 years old are less than or equal to 30 years old.There was a statistically significant difference in the expression of bFGF(0.046±0.001)in the 40-year-old group(P<0.05).b.Compared with the expression of bFGF(0.049±0.006)in the group of 50 years old or older and 60 years old,it was less than 18 years old.There was a statistically significant difference in the expression of bFGF between the 30-year-old group and the bFGF in the 30-year-old group and the 40-year-old group(P<0.05).c.There was no significant difference in HIF-1 and VEGF between the age groups(P>0.05).3)Comparison between the depressive disorder group and the normal control group:comparison of VEGF between the 1 group a.The VEGF expression of the depression group greater than or equal to 18 years old and less than 30 was less than the normal control group greater than or equal to 18 years old and less than 30 VEGF(1.764 ±0.762).(P<0.05);b.The expression of VEGF in the depressive disorder group was greater than or equal to 30 years old and less than 40 years old than that in the normal control group,which was greater than or equal to 30 years old and younger than 40 years old(1.581±0.676)expression(P<0.05);c.The expression of VEGF in the depression group was greater than or equal to 40 years old and less than 50 years old than the normal control group was greater than or equal to 40 years old and less than 50 years old VEGF(1.8711±0.956)expression(P<0.05);d.depression group was greater than or equal to 50 years old The expression of VEGF equal to 60 years old was lower than that of the normal control group,which was greater than or equal to 50 years old and less than or equal to 60 years old(P<0.05).The comparison of HIF1 between the two groups:a.The depression group was 18 or older and less than 30 years old.The expression of HIF1(1.461±0.802)was higher than that of the normal control group,which was greater than or equal to 18 years old and younger than 30 years old(1.097±0.581)(p<0.05);b.HIF1 was greater than or equal to 30 years old and younger than 40 years old.(1.707±0.830)expression is higher than normal control group,etc.The expression of HIF1(1.101 ±0.539)was less than 40 years old at 30 years old(P<0.05);c.The expression of HIF1(1.7761±0.714)in the depression group was greater than or equal to 40 years old and less than 50 years old than the normal control group was greater than or equal to 40 years old.The expression of HIF1(1.193 ± 0.472)in 50 years old(P<0.05);3There was no significant difference in HIF-1 expression between the depression group and the normal control group between 50 years old and 60 years old and no more than 60 years old.Significance(P>0.05).There was no significant difference in the expression of bFGF between the depressive disorder group and the normal age group(P>0.05).2.Basic research stage:1)Behavioral results:1 In the third week of the experiment,the horizontal and vertical scores of each group of open box experiments were compared:depression model group(19.50 ± 2.55)and(8.88±2.59),fluoxetine group(The scores of 23.13±7.30)and(9.50±1.93)were lower than those of the control group(54.13±6.29)and(15.86±3.44),and the differences were significant(P<0.01).2)Comparison of horizontal and vertical scores of open-box experiments in the 5th weekend of the experiment:fluoxetine group(29.62±2.83)and(13.25±1.28)were higher than depression model group(16.13±2.70)and(8.00±2.14).The differences were significant(P<0.01).3)Comparison of sucrose preference in the sucrose experiment of the third week of the experiment:depression model group(0.55±0.03)and fluoxetine group(0.54±0.03)were lower than the control group(0.76±0.04),the difference was significant(P<0.01).At the end of the 5th week,the sucrose preference of the sucrose test in each group was compared.The sucrose preference of the fluoxetine group(0.72±0.05)was higher than that of the depression model group(0.57±0.03),and the difference was significant(P<0.01).2)Western blot analysis:the expression of VEGF protein(0.80±0.10)in hippocampus of control group was higher than that of depression model group(0.44±0.08),the difference was significant(P<0.01);the VEGF protein of hippocampus in fluoxetine group(0.98±0.13)The expression was higher than that in the depression model group(P<0.01).The bFGF protein expression in the control group(0.86 ± 0.08)was higher than that in the depression model group(0.27 ± 0.04),and the difference was statistically significant(P<0.01).The expression of bFGF protein in hippocampus of fluoxetine group(1.10±0.13)was higher than that of bFGF protein in depression model group(P<0.01).3)RT-PCR assay:VEGF mRNA(1.80±0.25)expression in fluoxetine group was higher than that in depression model group(0.01±0.002),the difference was significant(P<0.01).The expression of VEGF mRNA(0.87±0.17)in hippocampus of control group was higher than that of VEGF mRNA in depression model group,the difference was statistically significant(P<0.01).The expression of bFGF mRNA(1.46±1.02)in the control group was significantly higher than that in the depression model group(0.002±0.0002),and the difference was statistically significant(P<0.01).The expression of bFGF mRNA(14.68±4.98)in the fluoxetine group was significantly higher than that in the depression group.The expression of bFGF mRNA in the model group was statistically significant(P<0.01)Conclusion:Clinical research conclusions:VEGF and HIF1 have different expression levels in patients with depression of different ages.With age,the expression changes are increased,suggesting that VEGF and HIF1 may be involved in the pathogenesis of depressive disorder,especially late onset depression.Changes in the expression of angiogenic factors,and even changes in angiogenesis,may be one of the important causes of depressive disorder.The relationship between basic fibroblast growth factor(bFGF)and the pathogenesis of depressive disorder is still unclear and needs further study.Basic research conclusions:Behavioral stage:Using CUMS,the behavioral scores of the depression model group were lower than the control group,suggesting that the rat depression model was successfully modeled.Molecular biology experimental stage:Western blot and RT-PCR experiments showed that VEGF,bFGF protein and gene expression were decreased in the depression model group. |
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