| Objective:Henoch-Sch?nlein purpura(HSP)is a common systemic vasculitis that often occurs in children aged 3-10 years old,but its pathogenesis is still unclear.The concentrations of HMGB1 and MyD88in peripheral blood of children with purpura nephritis group,Henoch-Sch?nlein purpura children without nephritis group and healthy group were measured,and the concentrations of HMGB1 and MyD88were compared between each groups to explore the correlation among HMGB1-MyD88 inflammatory pathway,the pathogenesis of HSP and purpura nephritis,so as to further reveal the pathogenesis of Henoch-Sch?nlein purpura.Methods:In this experiment,59 children with Henoch-Sch?nlein purpura who were diagnosed in Jining First People's Hospital were collected according to the inclusion and exclusion criteria,of which 16children with purpura nephritis were treated as purpura nephritis group(named HSPN group),and the remaining 43 children were treated as Henoch-Sch?nlein purpura children without nephritis group(named non-HSPN group).51 children from the Physical Examination Center in the same period were randomly selected as the control group.3ml of peripheral blood was collected from each subject,and their plasma HMGB1 and MyD88 concentrations were detected by ELISA.Then the differences of plasma HMGB1 and MyD88 concentrations among HSPN group,non-HSPN group and control group were compared,the correlation of HMGB1-MyD88 inflammatory pathway and the pathogenesis of Henoch-Sch?nlein purpura was observed.Results:(1)16 cases in the HSPN group,10 males and 6 females with an average age of(8.25±3.317)years;43 cases in the non-HSPN group,29males and 14 females with an average age of(7.53±2.814)years;51cases in the control group,29 males and 22 females with an average age of(6.16±3.252)years old.The comparison of gender in the three groups were?~2=1.110,P=0.574(P>0.05),the comparison of ages in the three groups were P=0.328(P>0.05),and there had not difference among the three groups.(2)In Henoch-Sch?nlein purpura children,the average concentration of male HMGB1 was(364.8324±94.58985)pg/ml,the average concentration of female HMGB1 was(397.2957±94.19997)pg/ml,there didn't have statistical significance with t=-1.250,P=0.217>0.05.The average concentration of male MyD88 was(4.7398±0.78956)ng/ml,the average concentration of female MyD88 was(4.5715±0.72256)ng/ml,there didn't have statistical significance with t=0.797,P=0.429>0.05.(3)The average concentration of plasma HMGB1 in the HSPN group was(443.2988±75.30543)pg/ml,the average concentration of plasma HMGB1 in the non-HSPN group was(350.7348±89.64883)pg/ml,and the average concentration of plasma HMGB1 in the control group was(198.6670±66.05788)pg/ml.The comparison of the three groups were P=0.000(P<0.05),and there was a significant difference in the average concentration of plasma HMGB1 among the three groups.There was a significant difference between HSPN group and control group(P=0.000<0.05),and the average concentration of plasma HMGB1 in HSPN group was higher than that in control group;there was a significant difference between non-HSPN group and control group(P=0.000<0.05),and the average concentration of plasma HMGB1 in non-HSPN group was higher than that in control group;there was a significant difference between HSPN group and non-HSPN group(P=0.000<0.05),and the average concentration of plasma HMGB1 in HSPN group was higher than that in non-HSPN group.(4)The average concentration of plasma MyD88 was(5.0600±0.57175)ng/ml in the HSPN group,(4.5423±0.78612)ng/ml in the non-HSPN group and(3.2148±0.54557)ng/ml in the control group.There was a significant difference in plasma concentration of MyD88among the three groups(P=0.000<0.05).There was a significant difference between HSPN group and control group(P=0.000<0.05),and the plasma MyD88 concentration in HSPN group was higher than that in control group;there was a significant difference between non-HSPN group and control group(P=0.000<0.05),and the plasma MyD88concentration in non-HSPN group was higher than that in control group;there was a significant difference between HSPN group and non-HSPN group(P=0.008<0.05),and the average plasma MyD88 concentration in HSPN group was higher than that in non-HSPN group.(5)There was a positive correlation between the average plasma concentration of HMGB1 and MyD88 in children with Henoch-Sch?nlein purpura(r=0.504,P=0.000<0.05).This positive correlation is stronger in children with HSPN group(r=0.504,P=0.000<0.05),And this positive correlation is weaker in children with non-HSPN group(r=0.504,P=0.000<0.05).Conclusion:In this experiment,no difference was found in the concentration of HMGB1 and MyD88 in different genders.The average concentration of plasma HMGB1,MyD88 in purpura nephritis children is higher than that of Henoch-Sch?nlein purpura children without renal damage,and both of them are higher than that of the control group.It is speculated that the significant increase of HMGB1,MyD88 are associated with purpura nephritis,that is,the significantly increased HMGB1,MyD88 may indicate purpura nephritis.There is a positive correlation between the plasma HMGB1 and MyD88 average concentration in children with Henoch-Sch?nlein purpura,and the positive correlation is stronger in purpura nephritis children,suggesting that HMGB1 may participate in the pathogenesis of Henoch-Sch?nlein purpura,through the classical Myd88 dependent inflammatory pathway,and in purpura nephritis,this inflammatory pathway is more powerful. |
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