Long Non-coding RNA Al022324.3 Supresses Proliferation And Migration Of Hepatoma Cells By Reversing EMT

In order to investigate the role of LncRNA AL022324.3 in the development of liver cancer,we conducted a series of experiments.The experimental results showed that we performed QPCR experiments to measure the gene silencing efficiency after transfection.The results showed that the gene silencing efficiency was the highest at 48 hours after transfection,and the silencing efficiency reached 75%.We performed the MTT test.The results showed that the OD value of the cells was significantly decreased compared with the control group after silencing the AL022324.3 gene,indicating that the cell proliferation was significantly inhibited,especially on the fourth day after transfection,the effect was most obvious.The OD value decreased by more than 25%.The scratch test showed that the silencing AL022324.3 gene was significantly shorter than the control group.After silencing the AL022324.3 gene,cell migration was observed.Invasion was significantly inhibited.Allogeneic tumor formation experiments in nude mice showed that after silencing AL022324.3 gene,the proliferation of tumors in nude mice was significantly inhibited compared with the control group.The cells were extracted from nude mice and subjected to real-time PCR.The results showed that in the AL022324.3 gene silencing group,the expression levels of EMT-related genes in vivo and in vitro changed,such as increased expression of E-cadherin and decreased expression of N-cadherin.Extracting proteins from HepG2,MHCC-97 H and xenografted tumor tissues in nude mice,Western blotting experiments showed that the expression of EMT-related proteins was consistent with the results of QPCR experiments,indicating that inhibition of the expression of AL022324.3 inhibited the proliferation,migration and invasion of hepatoma cells and tumor tissues by affecting the expression of EMT-related proteins.